| Literature DB >> 35779854 |
Naoki Matsumoto1, Nalin Singh1, Kin Sing Lee2, Bogdan Barnych1, Christophe Morisseau1, Bruce D Hammock3.
Abstract
The cellular mechanism by which epoxy fatty acids (EpFA) improves disease status is not well characterized. Previous studies suggest the involvement of cellular receptors and cyclic AMP (cAMP). Herein, the action of EpFAs derived from linoleic acid (LA), arachidonic acid (ARA), and docosahexaenoic acid on cAMP levels was studied in multiple cell types to elucidate relationships between EpFAs, receptors and cells' origin. cAMP levels were enhanced in HEK293 and LLC-PK1 cells by EpFAs from LA and ARA. Using selective antagonists, the EpFA effects on cAMP levels appear dependent on the prostaglandin E2 receptor 2 (EP2) but not 4 (EP4). Human coronary artery smooth muscle cells responded similarly to the EpFAs. However, we were not able to show the involvement of any of the receptors tested in this cell type. The results pinpointed distinct cell lines and receptor subtypes that natively respond to EpFA.Entities:
Keywords: Cyclic AMP; Dihydroxyeicosatrienoic acid; EP2; Eicosanoid; Epoxyeicosatrienoic acid; Inflammation; Soluble epoxide hydrolase
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Year: 2022 PMID： 35779854 PMCID： PMC9530012 DOI： 10.1016/j.prostaglandins.2022.106662
Source DB: PubMed Journal: Prostaglandins Other Lipid Mediat ISSN： 1098-8823 Impact factor: 3.813