| Literature DB >> 35776354 |
Constance A Sobsey1,2, Bjoern Froehlich3,4, Gerald Batist2,5,6, Christoph H Borchers7,8.
Abstract
The immuno-MALDI-MS method can be used to quantify low-abundance proteins from clinical samples that offer only a limited amount of material for analysis. An internal standard, in the form of a stable isotope-labeled peptide, is used to ensure reproducible and absolute quantitation. The protocol described here was optimized for the quantitation of AKT1 and AKT2, but we offer instructions on how to adapt the method to target other proteins. The described workflow is compatible with automation via a liquid handling robot for high-throughput applications.Entities:
Keywords: Absolute protein quantitation; Immunoenrichment; Internal peptide standard; MALDI; Proteomics; TOF mass spectrometry (MS); iMALDI
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Year: 2022 PMID： 35776354 DOI： 10.1007/978-1-0716-2409-8_13
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745