Literature DB >> 35776334

Humanized Mouse Model to Study the P2X7 Receptor in Graft-Versus-Host Disease.

Debbie Watson1,2, Sam R Adhikary3,4,5, Peter Cuthbertson3,4, Nicholas J Geraghty3,4, Katrina M Bird3,4, Amal Elhage3,4, Chloe Sligar3,4, Ronald Sluyter3,4.   

Abstract

Humanized mouse models of graft-versus-host disease (GVHD), where human immune cells are injected into immune deficient mice, are well established and provide opportunities to investigate pathways involved in GVHD development. This chapter provides an overview of human immune cell isolation, injection of these cells into immune deficient mice, monitoring of mice for signs of GVHD, and assessment of human cell engraftment using flow cytometry. Further, this chapter focuses on the P2X7 signaling pathway involved in GVHD, and describes a strategy to block the P2X7 receptor and examine the effect of this on GVHD development.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Flow cytometry; Hematopoietic stem cell transplantation; Human peripheral blood mononuclear cells; NSG mice; P2X7; Xenogeneic graft-versus-host disease

Mesh：

Substances：

Year:  2022        PMID： 35776334     DOI： 10.1007/978-1-0716-2384-8_18

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  25 in total

Review 1.  Humanized mouse models: Application to human diseases.

Authors:  Ryoji Ito; Takeshi Takahashi; Mamoru Ito
Journal:  J Cell Physiol       Date:  2017-07-04       Impact factor: 6.384

Review 2.  Insights into the pathogenesis of GvHD: what mice can teach us about man.

Authors:  J Hülsdünker; R Zeiser
Journal:  Tissue Antigens       Date:  2014-12-23

3.  Human lymphoid and myeloid cell development in NOD/LtSz-scid IL2R gamma null mice engrafted with mobilized human hemopoietic stem cells.

Authors:  Leonard D Shultz; Bonnie L Lyons; Lisa M Burzenski; Bruce Gott; Xiaohua Chen; Stanley Chaleff; Malak Kotb; Stephen D Gillies; Marie King; Julie Mangada; Dale L Greiner; Rupert Handgretinger
Journal:  J Immunol       Date:  2005-05-15       Impact factor: 5.422

4.  Polymorphism in Sirpa modulates engraftment of human hematopoietic stem cells.

Authors:  Katsuto Takenaka; Tatiana K Prasolava; Jean C Y Wang; Steven M Mortin-Toth; Sam Khalouei; Olga I Gan; John E Dick; Jayne S Danska
Journal:  Nat Immunol       Date:  2007-11-04       Impact factor: 25.606

5.  Identification of a nonsense mutation in the carboxyl-terminal region of DNA-dependent protein kinase catalytic subunit in the scid mouse.

Authors:  T Blunt; D Gell; M Fox; G E Taccioli; A R Lehmann; S P Jackson; P A Jeggo
Journal:  Proc Natl Acad Sci U S A       Date:  1996-09-17       Impact factor: 11.205

6.  Defective lymphoid development in mice lacking expression of the common cytokine receptor gamma chain.

Authors:  X Cao; E W Shores; J Hu-Li; M R Anver; B L Kelsall; S M Russell; J Drago; M Noguchi; A Grinberg; E T Bloom
Journal:  Immunity       Date:  1995-03       Impact factor: 31.745

7.  A new Hu-PBL model for the study of human islet alloreactivity based on NOD-scid mice bearing a targeted mutation in the IL-2 receptor gamma chain gene.

Authors:  Marie King; Todd Pearson; Leonard D Shultz; Jean Leif; Rita Bottino; Massimo Trucco; Mark A Atkinson; Clive Wasserfall; Kevan C Herold; Robert T Woodland; Madelyn R Schmidt; Bruce A Woda; Michael J Thompson; Aldo A Rossini; Dale L Greiner
Journal:  Clin Immunol       Date:  2007-12-21       Impact factor: 3.969

8.  Current Concepts and Advances in Graft-Versus-Host Disease Immunology.

Authors:  Geoffrey R Hill; Brian C Betts; Victor Tkachev; Leslie S Kean; Bruce R Blazar
Journal:  Annu Rev Immunol       Date:  2021-01-11       Impact factor: 28.527

9.  Human peripheral blood leucocyte non-obese diabetic-severe combined immunodeficiency interleukin-2 receptor gamma chain gene mouse model of xenogeneic graft-versus-host-like disease and the role of host major histocompatibility complex.

Authors:  M A King; L Covassin; M A Brehm; W Racki; T Pearson; J Leif; J Laning; W Fodor; O Foreman; L Burzenski; T H Chase; B Gott; A A Rossini; R Bortell; L D Shultz; D L Greiner
Journal:  Clin Exp Immunol       Date:  2009-07       Impact factor: 4.330
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