Literature DB >> 35771457

Multiplexing Seahorse XFe24 and ImageXpress® Nano Platforms for Comprehensive Evaluation of Mitochondrial Bioenergetic Profile and Neuronal Morphology.

Smijin K Soman1, Maryann Swain1, Ruben K Dagda2.   

Abstract

The measurement of mitochondrial function has become imperative to understand and characterize diseases characterized by bioenergetic alterations. The advancement of automation and application of high-throughput technologies has propelled our understanding of biological complexity and facilitated drug discovery. Seahorse extracellular flux (XFe) technology measures changes in dissolved oxygen and proton concentration in cell culture media, providing kinetic measurements of oxidative phosphorylation and glycolytic metabolism. ImageXpress® Nano is an automated fluorescent microscope with the ability to perform high-content, fast, and robust imaging in multi-well formats. In this chapter, we present a comprehensive protocol to multiplex the Seahorse XFe24 analyzer with the ImageXpress® Nano high content imaging microscope to provide a comprehensive yet rigorous profile of bioenergetics and its correlation to neuronal function and morphology.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Bioenergetics; ECAR; High-throughput imaging; Neurodegeneration; Neuron; OCR; Primary neuronal culture

Mesh：

Year:  2022        PMID： 35771457     DOI： 10.1007/978-1-0716-2309-1_26

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  8 in total

Review 1.  The oxidative phosphorylation system in mammalian mitochondria.

Authors:  Sergio Papa; Pietro Luca Martino; Giuseppe Capitanio; Antonio Gaballo; Domenico De Rasmo; Anna Signorile; Vittoria Petruzzella
Journal:  Adv Exp Med Biol       Date:  2012       Impact factor: 2.622

2.  Mitochondrial complex I deficiency in Parkinson's disease.

Authors:  A H Schapira; J M Cooper; D Dexter; J B Clark; P Jenner; C D Marsden
Journal:  J Neurochem       Date:  1990-03       Impact factor: 5.372

Review 3.  The Biochemistry and Physiology of Mitochondrial Fatty Acid β-Oxidation and Its Genetic Disorders.

Authors:  Sander M Houten; Sara Violante; Fatima V Ventura; Ronald J A Wanders
Journal:  Annu Rev Physiol       Date:  2015-10-14       Impact factor: 19.318

Review 4.  Mitochondrial diseases.

Authors:  Gráinne S Gorman; Patrick F Chinnery; Salvatore DiMauro; Michio Hirano; Yasutoshi Koga; Robert McFarland; Anu Suomalainen; David R Thorburn; Massimo Zeviani; Douglass M Turnbull
Journal:  Nat Rev Dis Primers       Date:  2016-10-20       Impact factor: 52.329

5.  Mitochondrial PKA Is Neuroprotective in a Cell Culture Model of Alzheimer's Disease.

Authors:  Tania Das Banerjee; Kelly Reihl; Maryann Swain; Mariana Torres; Ruben K Dagda
Journal:  Mol Neurobiol       Date:  2021-02-23       Impact factor: 5.682

6.  Inhibition of the mitochondrial calcium uniporter rescues dopaminergic neurons in pink1-/- zebrafish.

Authors:  Smijin Soman; Marcus Keatinge; Mahsa Moein; Marc Da Costa; Heather Mortiboys; Alexander Skupin; Sreedevi Sugunan; Michal Bazala; Jacek Kuznicki; Oliver Bandmann
Journal:  Eur J Neurosci       Date:  2016-12-28       Impact factor: 3.386

Review 7.  The MPTP Story.

Authors:  J William Langston
Journal:  J Parkinsons Dis       Date:  2017       Impact factor: 5.568

Review 8.  The genetics and pathology of mitochondrial disease.

Authors:  Charlotte L Alston; Mariana C Rocha; Nichola Z Lax; Doug M Turnbull; Robert W Taylor
Journal:  J Pathol       Date:  2016-11-02       Impact factor: 7.996
  8 in total

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