Literature DB >> 35771450

Measuring the Mitochondrial Ubiquinone (Q) Pool Redox State in Isolated Respiring Mitochondria.

Marten Szibor1,2, Estelle Heyne3, Carlo Viscomi4, Anthony L Moore5.   

Abstract

The ubiquinone (Q) pool represents a node in the mitochondrial electron transport chain (ETC) onto which the electrons of all respiratory dehydrogenases converge. The redox state of the Q pool correlates closely with the electron flux through the ETC and is thus a parameter of great metabolic value for both the mitochondrial and cellular metabolism. Here, we describe the simultaneous measurement of respiratory rates of isolated mouse heart mitochondria and the redox state of their Q pool using a custom-made combination of a Clark-type oxygen electrode and a Q electrode.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Mitochondria; Redox state; Respiratory rates; Ubiquinone pool

Mesh：

Substances：

Year:  2022        PMID： 35771450     DOI： 10.1007/978-1-0716-2309-1_19

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  9 in total

1.  Aging selectively decreases oxidative capacity in rat heart interfibrillar mitochondria.

Authors:  S W Fannin; E J Lesnefsky; T J Slabe; M O Hassan; C L Hoppel
Journal:  Arch Biochem Biophys       Date:  1999-12-15       Impact factor: 4.013

2.  New insights into the regulation of plant succinate dehydrogenase. On the role of the protonmotive force.

Authors:  C Affourtit; K Krab; G R Leach; D G Whitehouse; A L Moore
Journal:  J Biol Chem       Date:  2001-05-11       Impact factor: 5.157

3.  Separation of mitochondria into two morphologically and biochemically distinct types.

Authors:  A K LAIRD; O NYGAARD; H RIS; A D BARTON
Journal:  Exp Cell Res       Date:  1953-09       Impact factor: 3.905

4.  Functional expression of the plant alternative oxidase affects growth of the yeast Schizosaccharomyces pombe.

Authors:  C Affourtit; M S Albury; K Krab; A L Moore
Journal:  J Biol Chem       Date:  1999-03-05       Impact factor: 5.157

5.  Regulation of thermogenesis in flowering Araceae: the role of the alternative oxidase.

Authors:  Anneke M Wagner; Klaas Krab; Marijke J Wagner; Anthony L Moore
Journal:  Biochim Biophys Acta       Date:  2008-04-09

6.  Measurement of the redox state of the ubiquinone pool in Rhodobacter capsulatus membrane fragments.

Authors:  D Zannoni; A L Moore
Journal:  FEBS Lett       Date:  1990-10-01       Impact factor: 4.124

7.  The relationship between electron flux and the redox poise of the quinone pool in plant mitochondria. Interplay between quinol-oxidizing and quinone-reducing pathways.

Authors:  C W Van den Bergen; A M Wagner; K Krab; A L Moore
Journal:  Eur J Biochem       Date:  1994-12-15

8.  Restoration of electron transport without proton pumping in mammalian mitochondria.

Authors:  Ester Perales-Clemente; Maria Pilar Bayona-Bafaluy; Acisclo Pérez-Martos; Antoni Barrientos; Patricio Fernández-Silva; Jose Antonio Enriquez
Journal:  Proc Natl Acad Sci U S A       Date:  2008-11-19       Impact factor: 11.205

9.  Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers.

Authors:  Hélène Lemieux; Pierre U Blier; Erich Gnaiger
Journal:  Sci Rep       Date:  2017-06-06       Impact factor: 4.379
  9 in total

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