Literature DB >> 35771436

Measurement of Mitochondrial (Dys)Function in Cellular Systems Using Electron Paramagnetic Resonance (EPR): Oxygen Consumption Rate and Superoxide Production.

Donatienne d'Hose1, Bernard Gallez2.   

Abstract

The oxygen consumption rate (OCR) and superoxide production are crucial when assessing mitochondrial function and/or dysfunction. EPR spectroscopy allows the measurement of both components either independently or simultaneously in a same cellular or mitochondrial preparation. OCR determination using EPR oximetry is based on the change in EPR linewidth of a paramagnetic oxygen sensing probe (a perdeuterated nitroxide) in the presence of oxygen consuming cells in a closed system. Superoxide production can be monitored by the oxidation of cyclic hydroxylamines into nitroxides. The contribution of superoxide to the nitroxide formation is deduced from experiments in the presence and in the absence of SOD and PEG-SOD as appropriate controls.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  EPR; ETC; Hydroxylamine; Mitochondrial function; Nitroxide; Oximetry; Oxygen consumption rate (OCR); Superoxide

Mesh：

Substances：

Year:  2022        PMID： 35771436     DOI： 10.1007/978-1-0716-2309-1_5

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  15 in total

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Authors:  Nathalie Crokart; Bénédicte F Jordan; Christine Baudelet; Gregory O Cron; Julie Hotton; Kim Radermacher; Vincent Grégoire; Nelson Beghein; Philippe Martinive; Caroline Bouzin; Olivier Feron; Bernard Gallez
Journal:  Clin Cancer Res       Date:  2007-01-15       Impact factor: 12.531

2.  Comparison of methods for measuring oxygen consumption in tumor cells in vitro.

Authors:  Caroline Diepart; Julien Verrax; Pedro Buc Calderon; Olivier Feron; Bénédicte F Jordan; Bernard Gallez
Journal:  Anal Biochem       Date:  2009-09-18       Impact factor: 3.365

3.  A mitochondrial switch promotes tumor metastasis.

Authors:  Paolo E Porporato; Valéry L Payen; Jhudit Pérez-Escuredo; Christophe J De Saedeleer; Pierre Danhier; Tamara Copetti; Suveera Dhup; Morgane Tardy; Thibaut Vazeille; Caroline Bouzin; Olivier Feron; Carine Michiels; Bernard Gallez; Pierre Sonveaux
Journal:  Cell Rep       Date:  2014-07-24       Impact factor: 9.423

4.  Mechanism of reoxygenation after antiangiogenic therapy using SU5416 and its importance for guiding combined antitumor therapy.

Authors:  Réginald Ansiaux; Christine Baudelet; Bénédicte F Jordan; Nathalie Crokart; Philippe Martinive; Julie DeWever; Vincent Grégoire; Olivier Feron; Bernard Gallez
Journal:  Cancer Res       Date:  2006-10-01       Impact factor: 12.701

5.  Arsenic trioxide treatment decreases the oxygen consumption rate of tumor cells and radiosensitizes solid tumors.

Authors:  Caroline Diepart; Oussama Karroum; Julie Magat; Olivier Feron; Julien Verrax; Pedro Buc Calderon; Vincent Grégoire; Philippe Leveque; Julie Stockis; Nicolas Dauguet; Bénédicte F Jordan; Bernard Gallez
Journal:  Cancer Res       Date:  2011-12-02       Impact factor: 12.701

6.  A Fast Hydrogen Sulfide-Releasing Donor Increases the Tumor Response to Radiotherapy.

Authors:  Géraldine De Preter; Caroline Deriemaeker; Pierre Danhier; Lucie Brisson; Thanh Trang Cao Pham; Vincent Grégoire; Bénédicte F Jordan; Pierre Sonveaux; Bernard Gallez
Journal:  Mol Cancer Ther       Date:  2015-12-18       Impact factor: 6.261

7.  Early reoxygenation in tumors after irradiation: determining factors and consequences for radiotherapy regimens using daily multiple fractions.

Authors:  Nathalie Crokart; Bénédicte F Jordan; Christine Baudelet; Reginald Ansiaux; Pierre Sonveaux; Vincent Grégoire; Nelson Beghein; Julie DeWever; Caroline Bouzin; Olivier Feron; Bernard Gallez
Journal:  Int J Radiat Oncol Biol Phys       Date:  2005-11-01       Impact factor: 7.038

8.  Nitric oxide as a radiosensitizer: evidence for an intrinsic role in addition to its effect on oxygen delivery and consumption.

Authors:  Bénédicte F Jordan; Pierre Sonveaux; Olivier Feron; Vincent Grégoire; Nelson Beghein; Chantal Dessy; Bernard Gallez
Journal:  Int J Cancer       Date:  2004-05-01       Impact factor: 7.396

9.  Insulin increases the sensitivity of tumors to irradiation: involvement of an increase in tumor oxygenation mediated by a nitric oxide-dependent decrease of the tumor cells oxygen consumption.

Authors:  Bénédicte F Jordan; Vincent Grégoire; Roger J Demeure; Pierre Sonveaux; Olivier Feron; Julia O'Hara; Valérie P Vanhulle; Nathalie Delzenne; Bernard Gallez
Journal:  Cancer Res       Date:  2002-06-15       Impact factor: 12.701

10.  Influence of cell detachment on the respiration rate of tumor and endothelial cells.

Authors:  Pierre Danhier; Tamara Copetti; Géraldine De Preter; Philippe Leveque; Olivier Feron; Bénédicte F Jordan; Pierre Sonveaux; Bernard Gallez
Journal:  PLoS One       Date:  2013-01-30       Impact factor: 3.240
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