| Literature DB >> 35768498 |
Chao Wei1,2,3, Lumeng Jia4, Xiaona Huang1,2,3, Jin Tan1,2,3, Mulan Wang1,2,3, Jing Niu5, Yingping Hou6, Jun Sun1,2,3, Pengguihang Zeng1,2,3,7, Jia Wang1,2,3, Li Qing1,2,3, Lin Ma1,2,3, Xinyi Liu1,2,3, Xiuxiao Tang1,2,3, Fenjie Li1,2,3,8, Shaoshuai Jiang1,2,3, Jingxin Liu1,2,3, Tingting Li9, Lili Fan10, Yujie Sun4,11, Juntao Gao12, Cheng Li13,14, Junjun Ding15,16,17.
Abstract
Chromatin is spatially organized into three-dimensional structures at different levels including A/B compartments, topologically associating domains and loops. The canonical CTCF-mediated loop extrusion model can explain the formation of loops. However, the organization mechanisms underlying long-range chromatin interactions such as interactions between A-A compartments are still poorly understood. Here we show that different from the canonical loop extrusion model, RYBP-mediated phase separation of CTCF organizes inter-A compartment interactions. Based on this model, we designed and verified an induced CTCF phase separation system in embryonic stem cells (ESCs), which facilitated inter-A compartment interactions, improved self-renewal of ESCs and inhibited their differentiation toward neural progenitor cells. These findings support a novel and non-canonical role of CTCF in organizing long-range chromatin interactions via phase separation.Entities:
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Year: 2022 PMID： 35768498 PMCID： PMC9343660 DOI： 10.1038/s41422-022-00676-0
Source DB: PubMed Journal: Cell Res ISSN： 1001-0602 Impact factor: 46.297