Peng Cao1, Chao Tang1,2, Xiao Wu1, Ming Qian1, Shouzheng Lv1, Hongru Gao1, Xin Qiao1, Guodong Chen1,3, Peng Wang1, Shaoling Zhang1, Juyou Wu4,5,6. 1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Centre of Pear Engineering Technology Research, Nanjing Agricultural University, Nanjing, 210095, China. 2. Sanya Institute of Nanjing Agricultural University, Sanya, 572024, China. 3. College of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai'an, 223003, China. 4. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Centre of Pear Engineering Technology Research, Nanjing Agricultural University, Nanjing, 210095, China. juyouwu@njau.edu.cn. 5. Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing, 210014, China. juyouwu@njau.edu.cn. 6. Sanya Institute of Nanjing Agricultural University, Sanya, 572024, China. juyouwu@njau.edu.cn.
Abstract
MAIN CONCLUSION: Identification of CalS genes in seven Rosaceae species and functional characterization of PbrCalS5 in pear pollen tube growth by regulating callose deposition. Callose exists widely in angiosperms and has significant functions in a range of developmental processes. Callose is synthesized by callose synthase (CalS). However, the members of the callose synthase gene family and their evolutionary profiles, along with their biological functions, in species of the Rosaceae remain unknown. In this study, a total of 69 members of the CalS gene family in seven Rosaceae species (Fragaria vesca, Malus × domestica, Prunus avium, Pyrus bretschneideri, Prunus mume, Prunus persica and Rubus occidentalis) were identified and divided into six clades. Different types of gene duplication events contributed to the expansions of the CalS gene family in the seven species, with purifying selection playing a key role in the evolution of the CalS genes. Tissue-specific expression patterns analysis revealed that PbrCalS5 was highly expressed in the pear pollen tube and was selected for further functional analysis. Subcellular localization indicated that PbrCalS5 was localized in the plasma membrane and cell wall. Antisense oligodeoxynucleotide (AS-ODN) assays resulted in the inhibition of PbrCalS5 expression, leading to the decreased callose deposition in the pollen tube wall and subsequent inhibition of pear pollen tube growth. These results provide the theoretical basis for exploring the functional roles of CalS genes in pear pollen tube growth.
MAIN CONCLUSION: Identification of CalS genes in seven Rosaceae species and functional characterization of PbrCalS5 in pear pollen tube growth by regulating callose deposition. Callose exists widely in angiosperms and has significant functions in a range of developmental processes. Callose is synthesized by callose synthase (CalS). However, the members of the callose synthase gene family and their evolutionary profiles, along with their biological functions, in species of the Rosaceae remain unknown. In this study, a total of 69 members of the CalS gene family in seven Rosaceae species (Fragaria vesca, Malus × domestica, Prunus avium, Pyrus bretschneideri, Prunus mume, Prunus persica and Rubus occidentalis) were identified and divided into six clades. Different types of gene duplication events contributed to the expansions of the CalS gene family in the seven species, with purifying selection playing a key role in the evolution of the CalS genes. Tissue-specific expression patterns analysis revealed that PbrCalS5 was highly expressed in the pear pollen tube and was selected for further functional analysis. Subcellular localization indicated that PbrCalS5 was localized in the plasma membrane and cell wall. Antisense oligodeoxynucleotide (AS-ODN) assays resulted in the inhibition of PbrCalS5 expression, leading to the decreased callose deposition in the pollen tube wall and subsequent inhibition of pear pollen tube growth. These results provide the theoretical basis for exploring the functional roles of CalS genes in pear pollen tube growth.
Authors: Stephen A Bustin; Vladimir Benes; Jeremy A Garson; Jan Hellemans; Jim Huggett; Mikael Kubista; Reinhold Mueller; Tania Nolan; Michael W Pfaffl; Gregory L Shipley; Jo Vandesompele; Carl T Wittwer Journal: Clin Chem Date: 2009-02-26 Impact factor: 8.327
Authors: Barend H J de Graaf; Jason J Rudd; Michael J Wheeler; Ruth M Perry; Elizabeth M Bell; Kim Osman; F Christopher H Franklin; Vernonica E Franklin-Tong Journal: Nature Date: 2006-11-05 Impact factor: 49.962
Authors: C M Douglas; F Foor; J A Marrinan; N Morin; J B Nielsen; A M Dahl; P Mazur; W Baginsky; W Li; M el-Sherbeini Journal: Proc Natl Acad Sci U S A Date: 1994-12-20 Impact factor: 11.205