| Literature DB >> 35761040 |
Jiamei Liu1, Xuechen Li2.
Abstract
Serine/threonine ligation (STL) and cysteine/penicillamine ligation (CPL) are highly chemo- and regioselective reactions between unprotected peptides with C-terminus salicylaldehyde esters and unprotected peptides with N-terminus serine/threonine or cysteine/penicillamine, which serve as powerful tools for cyclic peptide natural product and chemical protein synthesis. Herein, we introduce the preparation of C-terminal peptide salicylaldehyde esters, serine/threonine ligation, cysteine/penicillamine ligation, and subsequent acidolysis.Entities:
Keywords: Acidolysis; Chemical protein synthesis; Cyclic peptide; Cysteine/penicillamine ligation; Peptide salicylaldehyde ester; Serine/threonine ligation
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Year: 2022 PMID： 35761040 DOI： 10.1007/978-1-0716-2489-0_3
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745