| Literature DB >> 3575503 |
L Morelli, P S Cocconcelli, V Bottazzi, G Damiani, L Ferretti, V Sgaramella.
Abstract
A method for the transformation of Lactobacillus protoplasts by plasmid DNA is reported. The procedure involves polyethylene glycol treatment of protoplasts to induce DNA uptake. A transformation efficiency ranging from 5 to 1000 transformants per microgram of DNA is achieved; the efficiency of protoplast regeneration ranged from 10 to 20%.Entities:
Mesh：
Substances：
Year: 1987 PMID： 3575503 DOI： 10.1016/0147-619x(87)90013-8
Source DB: PubMed Journal: Plasmid ISSN： 0147-619X Impact factor: 3.466