João Marcos G Barbosa1, Débora Ribeiro de Mendonça2, Lurian C David3, Taynara C E Silva3, Danielly A Fortuna Lima3, Anselmo E de Oliveira4, Welber Daniel Zanetti Lopes5, Maria Clorinda S Fioravanti2, Paulo H Jorge da Cunha2, Nelson R Antoniosi Filho6. 1. Laboratório de Métodos de Extração e Separação (LAMES), Instituto de Química (IQ), Universidade Federal de Goiás (UFG), Campus II - Samambaia, Goiânia, GO, 74690-900, Brazil. joaomarcosquim.ufg@outlook.com. 2. Escola de Veterinária e Zootecnia (EVZ), Universidade Federal de Goiás (UFG), Rodovia Goiânia - Nova Veneza, Km 8, Campus II - Samambaia, Goiânia, GO, CEP, 74001-970, Brazil. 3. Laboratório de Métodos de Extração e Separação (LAMES), Instituto de Química (IQ), Universidade Federal de Goiás (UFG), Campus II - Samambaia, Goiânia, GO, 74690-900, Brazil. 4. Laboratory of Theoretical and Computational Chemistry, Instituto de Química, UFG, Goiânia, GO, 74690-970, Brazil. 5. Centro de Parasitologia Veterinária, Escola de Veterinária e Zootecnia (EVZ), Universidade Federal de Goiás (UFG), Rodovia Goiânia - Nova Veneza, Km 8, Campus II - Samambaia, Goiânia, Goiás, CEP, 74001-970, Brazil. 6. Laboratório de Métodos de Extração e Separação (LAMES), Instituto de Química (IQ), Universidade Federal de Goiás (UFG), Campus II - Samambaia, Goiânia, GO, 74690-900, Brazil. nelsonroberto@ufg.br.
Abstract
INTRODUCTION: Trypanosomiasis caused by Trypanosoma vivax (T. vivax, subgenus Duttonella) is a burden disease in bovines that induces losses of billions of dollars in livestock activity worldwide. To control the disease, the first step is identifying the infected animals at early stages. However, convention tools for animal infection detection by T. vivax present some challenges, facilitating the spread of the pathogenesis. OBJECTIVES: This work aims to develop a new procedure to identify infected bovines by T. vivax using cerumen (earwax) in a volatilomic approach, here named cerumenolomic, which is performed in an easy, quick, accurate, and non-invasive manner. METHODS: Seventy-eight earwax samples from Brazilian Curraleiro Pé-Duro calves were collected in a longitudinal study protocol during health and inoculated stages. The samples were analyzed using Headspace/Gas Chromatography-Mass Spectrometry followed by multivariate analysis approaches. RESULTS: The cerumen analyses lead to the identification of a broad spectrum of volatile organic metabolites (VOMs), of which 20 VOMs can discriminate between healthy and infected calves (AUC = 0.991, sensitivity = 0.967, specificity = 1.000). Furthermore, 13 VOMs can indicate a pattern of discrimination between the acute and chronic phases of the T. vivax infection in the animals (AUC = 0.989, sensitivity = 0.944, specificity = 1.000). CONCLUSION: The cerumen volatile metabolites present alterations in their occurrence during the T.vivax infection, which may lead to identifying the infection in the first weeks of inoculation and discriminating between the acute and chronic phases of the illness. These results may be a breakthrough to avoid the T. vivax outbreak and provide a faster clinical approach to the animal.
INTRODUCTION: Trypanosomiasis caused by Trypanosoma vivax (T. vivax, subgenus Duttonella) is a burden disease in bovines that induces losses of billions of dollars in livestock activity worldwide. To control the disease, the first step is identifying the infected animals at early stages. However, convention tools for animal infection detection by T. vivax present some challenges, facilitating the spread of the pathogenesis. OBJECTIVES: This work aims to develop a new procedure to identify infected bovines by T. vivax using cerumen (earwax) in a volatilomic approach, here named cerumenolomic, which is performed in an easy, quick, accurate, and non-invasive manner. METHODS: Seventy-eight earwax samples from Brazilian Curraleiro Pé-Duro calves were collected in a longitudinal study protocol during health and inoculated stages. The samples were analyzed using Headspace/Gas Chromatography-Mass Spectrometry followed by multivariate analysis approaches. RESULTS: The cerumen analyses lead to the identification of a broad spectrum of volatile organic metabolites (VOMs), of which 20 VOMs can discriminate between healthy and infected calves (AUC = 0.991, sensitivity = 0.967, specificity = 1.000). Furthermore, 13 VOMs can indicate a pattern of discrimination between the acute and chronic phases of the T. vivax infection in the animals (AUC = 0.989, sensitivity = 0.944, specificity = 1.000). CONCLUSION: The cerumen volatile metabolites present alterations in their occurrence during the T.vivax infection, which may lead to identifying the infection in the first weeks of inoculation and discriminating between the acute and chronic phases of the illness. These results may be a breakthrough to avoid the T. vivax outbreak and provide a faster clinical approach to the animal.
Authors: João Marcos G Barbosa; Milenna K Fernandes Rodrigues; Lurian C David; Taynara C E Silva; Danielly A Fortuna Lima; Naiara Z Pereira; Emmanuel B D'Alessandro; Anselmo E de Oliveira; Paulo H Jorge da Cunha; Maria Clorinda S Fioravanti; Nelson R Antoniosi Filho Journal: Biomed Chromatogr Date: 2020-07-17 Impact factor: 1.902