Pei-Shin Hu1,2,3, Ning-Yi Hsia3,4, Wei-Ching Chien5, Mei-Chin Mong6, Te-Chun Hsia3,7, Heng-Ming Chang8, Yun-Chi Wang3, Wen-Shin Chang1,3, DA-Tian Bau9,3,10, Chia-Wen Tsai9,3. 1. Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan, R.O.C. 2. Department of Ophthalmology, Changhua Christian Hospital, Changhua, Taiwan, R.O.C. 3. Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan, R.O.C. 4. Department of Ophthalmology, China Medical University Hospital, Taichung, Taiwan, R.O.C. 5. Cell Therapy Center, Chang Bing Show Chwan Memorial Hospital, Changhua, Taiwan, R.O.C. 6. Department of Food Nutrition and Health Biotechnology, Asia University, Taichung, Taiwan, R.O.C. 7. The Ph.D. Program for Health Science and Industry, China Medical University, Taichung, Taiwan, R.O.C. 8. Department of Dentistry, Chang Bing Show Chwan Memorial Hospital, Changhua, Taiwan, R.O.C. 9. Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan, R.O.C.; artbau2@gmail.com wenwen816@gmail.com. 10. Department of Bioinformatics and Medical Engineering, Asia University, Taichung, Taiwan, R.O.C.
Abstract
BACKGROUND: The role of epigenetic alterations in the pathogenesis of retinal degenerative diseases, such as macular degeneration is not well established. This study aimed to evaluate whether treatments with gamma-mangostin can rescue the hydrogen peroxide (H2O2)-induced cytotoxicity in human retinal pigment epithelial (ARPE-19) cells. MATERIALS AND METHODS: ARPE-19 cells were treated with H2O2 alone or with gamma-mangostin plus H2O2 to investigate changes relating to cell viability, appearance of sub-G1 cells, antioxidant enzymes, and apoptotic-related proteins. RESULTS: The data showed that under H2O2 treatment of 400 μM, there was a significant decrease in cell viability and enhanced apoptosis, together with an increased expression of Bax, Bad, cleaved-caspase-3, -8, and -9 at the protein level. On the contrary, the protein expression levels of Bcl2 and Bcl-xl were decreased. Gamma-mangostin pre-treatments (2-16 μM) could effectively prevent all alterations. CONCLUSION: Gamma-mangostin may conduct its eye-protective effects against H2O2-induced oxidative damage via anti- apoptotic and antioxidant mechanisms in ARPE-19 cells.
BACKGROUND: The role of epigenetic alterations in the pathogenesis of retinal degenerative diseases, such as macular degeneration is not well established. This study aimed to evaluate whether treatments with gamma-mangostin can rescue the hydrogen peroxide (H2O2)-induced cytotoxicity in human retinal pigment epithelial (ARPE-19) cells. MATERIALS AND METHODS: ARPE-19 cells were treated with H2O2 alone or with gamma-mangostin plus H2O2 to investigate changes relating to cell viability, appearance of sub-G1 cells, antioxidant enzymes, and apoptotic-related proteins. RESULTS: The data showed that under H2O2 treatment of 400 μM, there was a significant decrease in cell viability and enhanced apoptosis, together with an increased expression of Bax, Bad, cleaved-caspase-3, -8, and -9 at the protein level. On the contrary, the protein expression levels of Bcl2 and Bcl-xl were decreased. Gamma-mangostin pre-treatments (2-16 μM) could effectively prevent all alterations. CONCLUSION: Gamma-mangostin may conduct its eye-protective effects against H2O2-induced oxidative damage via anti- apoptotic and antioxidant mechanisms in ARPE-19 cells.
Authors: Bilal Bin Hafeez; Ala Mustafa; Joseph W Fischer; Ashok Singh; Weixiong Zhong; Mohammed Ozair Shekhani; Louise Meske; Thomas Havighurst; KyungMann Kim; Ajit Kumar Verma Journal: Antioxid Redox Signal Date: 2014-02-06 Impact factor: 8.401
Authors: Lars G Fritsche; Wilmar Igl; Jessica N Cooke Bailey; Felix Grassmann; Sebanti Sengupta; Jennifer L Bragg-Gresham; Kathryn P Burdon; Scott J Hebbring; Cindy Wen; Mathias Gorski; Ivana K Kim; David Cho; Donald Zack; Eric Souied; Hendrik P N Scholl; Elisa Bala; Kristine E Lee; David J Hunter; Rebecca J Sardell; Paul Mitchell; Joanna E Merriam; Valentina Cipriani; Joshua D Hoffman; Tina Schick; Yara T E Lechanteur; Robyn H Guymer; Matthew P Johnson; Yingda Jiang; Chloe M Stanton; Gabriëlle H S Buitendijk; Xiaowei Zhan; Alan M Kwong; Alexis Boleda; Matthew Brooks; Linn Gieser; Rinki Ratnapriya; Kari E Branham; Johanna R Foerster; John R Heckenlively; Mohammad I Othman; Brendan J Vote; Helena Hai Liang; Emmanuelle Souzeau; Ian L McAllister; Timothy Isaacs; Janette Hall; Stewart Lake; David A Mackey; Ian J Constable; Jamie E Craig; Terrie E Kitchner; Zhenglin Yang; Zhiguang Su; Hongrong Luo; Daniel Chen; Hong Ouyang; Ken Flagg; Danni Lin; Guanping Mao; Henry Ferreyra; Klaus Stark; Claudia N von Strachwitz; Armin Wolf; Caroline Brandl; Guenther Rudolph; Matthias Olden; Margaux A Morrison; Denise J Morgan; Matthew Schu; Jeeyun Ahn; Giuliana Silvestri; Evangelia E Tsironi; Kyu Hyung Park; Lindsay A Farrer; Anton Orlin; Alexander Brucker; Mingyao Li; Christine A Curcio; Saddek Mohand-Saïd; José-Alain Sahel; Isabelle Audo; Mustapha Benchaboune; Angela J Cree; Christina A Rennie; Srinivas V Goverdhan; Michelle Grunin; Shira Hagbi-Levi; Peter Campochiaro; Nicholas Katsanis; Frank G Holz; Frédéric Blond; Hélène Blanché; Jean-François Deleuze; Robert P Igo; Barbara Truitt; Neal S Peachey; Stacy M Meuer; Chelsea E Myers; Emily L Moore; Ronald Klein; Michael A Hauser; Eric A Postel; Monique D Courtenay; Stephen G Schwartz; Jaclyn L Kovach; William K Scott; Gerald Liew; Ava G Tan; Bamini Gopinath; John C Merriam; R Theodore Smith; Jane C Khan; Humma Shahid; Anthony T Moore; J Allie McGrath; Reneé Laux; Milam A Brantley; Anita Agarwal; Lebriz Ersoy; Albert Caramoy; Thomas Langmann; Nicole T M Saksens; Eiko K de Jong; Carel B Hoyng; Melinda S Cain; Andrea J Richardson; Tammy M Martin; John Blangero; Daniel E Weeks; Bal Dhillon; Cornelia M van Duijn; Kimberly F Doheny; Jane Romm; Caroline C W Klaver; Caroline Hayward; Michael B Gorin; Michael L Klein; Paul N Baird; Anneke I den Hollander; Sascha Fauser; John R W Yates; Rando Allikmets; Jie Jin Wang; Debra A Schaumberg; Barbara E K Klein; Stephanie A Hagstrom; Itay Chowers; Andrew J Lotery; Thierry Léveillard; Kang Zhang; Murray H Brilliant; Alex W Hewitt; Anand Swaroop; Emily Y Chew; Margaret A Pericak-Vance; Margaret DeAngelis; Dwight Stambolian; Jonathan L Haines; Sudha K Iyengar; Bernhard H F Weber; Gonçalo R Abecasis; Iris M Heid Journal: Nat Genet Date: 2015-12-21 Impact factor: 38.330