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Literature DB >> 35737160

The Level of LINE-1 mRNA Is Increased in Extracellular Circulating Plasma RNA in Patients with Colorectal Cancer.

M L Filipenko¹, U A Boyarskikh², L S Leskov³, K V Subbotina², E A Khrapov², A V Sokolov⁴, I S Stilidi⁵, N E Kushlinskii⁵.

Abstract

We performed a comparative quantitative analysis of LINE-1 mRNA levels in extracellular total plasma RNA in patients with colon cancer and practically healthy donors. Quantitative multiplex PCR with reverse transcription was used to assess the level of LINE-1 and 18S rRNA mRNA in extracellular total plasma RNA. The median of LINE-1 mRNA values in colon cancer patients (4.95) was significantly higher than in healthy donors (2.3) (p=0.037). It was shown for the first time that the level of LINE-1 mRNA in total RNA from blood plasma can be determined in the format of a liquid biopsy and serve as a new potential non-invasive marker of colon cancer.

Entities: Chemical

Keywords: LINE-1; colorectal cancer; freely circulating RNA; long dispersed nucleotide repeats (LINE); transcription

Mesh：

Substances：

Year: 2022 PMID： 35737160 DOI： 10.1007/s10517-022-05530-2

Source DB: PubMed Journal: Bull Exp Biol Med ISSN： 0007-4888 Impact factor: 0.804

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The Level of LINE-1 mRNA Is Increased in Extracellular Circulating Plasma RNA in Patients with Colorectal Cancer.

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Review 6. Long Interspersed Element-1 Methylation Level as a Prognostic Biomarker in Gastrointestinal Cancers.

7. L1 retrotransposition occurs mainly in embryogenesis and creates somatic mosaicism.

Review 8. Regulation of transposable elements by DNA modifications.

Review 9. Evolutionary conflicts between viruses and restriction factors shape immunity.

10. Enhanced expression of LINE-1-encoded ORF2 protein in early stages of colon and prostate transformation.