High Concentration of Fermentable Sugars Prepared from Steam Exploded Lignocellulose in Periodic Peristalsis Integrated Fed-Batch Enzymatic Hydrolysis.

High concentrations of fermentable sugars are a demand for economical bioethanol production. A single process strategy cannot comprehensively solve the limiting factors in high-solid enzymatic hydrolysis. The multiple intensification strategies in this study achieved the goal of preparing high-concentration fermentable sugars of corn stalk with high solid loading and low enzyme loading. First, steam explosion pretreatment enhanced the hydrophilicity of substrates and enzymatic accessibility. Second, periodic peristalsis was used to improve the mass transfer efficiency and short the liquefaction time. Additionally, fed-batch feeding and enzyme reduced the enzyme loading. Ultimately, the intensification strategies above showed that the highest fermentable sugar content was 313.8 g/L with a solids loading as much as 50% (w/w) and enzyme loading as low as 12.5 FPU/g DM. Thus, these multiple intensification strategies were promising in the high-solid enzymatic hydrolysis of steam-exploded lignocellulose.