| Literature DB >> 35727440 |
Xiaofang Huang1,2, Liangting Xu1,2, Chuyun Bi1,2, Lili Zhao1,2, Limei Zhang1,2, Xuanyang Chen1,2, Shiqian Qi3, Shiqiang Lin4,5.
Abstract
Overlap extension PCR is one of the routinely used methods to generate mutagenic genes for the functional and structural study of proteins. However, it is time-consuming to design the overlapping mutagenic primers and gene primers by manual operation. In this chapter, we present a Python script that is able to search all the possible primer combinations according to the preset definitions and calculate the necessary parameters of each primer for the users, which could facilitate the primer design process. Up to 256 pairs of primers can be provided for selection using this script.Entities:
Keywords: Overlap extension PCR; Overlapping mutagenic primers; Primer design; Site-directed mutagenesis
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Year: 2022 PMID： 35727440 DOI： 10.1007/978-1-0716-2152-3_1
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745