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Literature DB >> 357268

Application of R-plasmid DNA's from Escherichia coli minicells in genetic transformation.

Abstract

Components of minicell lyzates of Escherichia coli P678.54 (R1 drd 19) and escherichia coli P678.54 (R6K) were visualized in an electron microscope and used for the transformation of Escherichia coli JC7623. The frequency of the resulting transformants (of the order of 10(-6)%) was not appreciably influenced by the manner of lyzate preparation. The presence of covalently closed circular DNA was detected in two different transformants using radioisotopes, thus demonstrating an autonomous existence of R1 drd 19 or R6K plasmids in tested transformants. This finding corresponds with the results of their genetic analysis.

Entities: Mutation Species

Mesh：

Substances：

Year: 1978 PMID： 357268 DOI： 10.1007/bf02876681

Source DB: PubMed Journal: Folia Microbiol (Praha) ISSN： 0015-5632 Impact factor: 2.099

23 in total

1. Dispersive transfer of the parental DNA molecule to the progeny of phage phiX-174.

Authors: A W KOZINSKI; W SZYBALSKI
Journal: Virology Date: 1959-10 Impact factor: 3.616

2. General method for the isolation of plasmid deoxyribonucleic acid.

Authors: P Guerry; D J LeBlanc; S Falkow
Journal: J Bacteriol Date: 1973-11 Impact factor: 3.490

3. Segregation into and replication of plasmid deoxyribonucleic acid in chromosomeless segregants of Escherichia coli.

Authors: J Inselburg
Journal: J Bacteriol Date: 1970-06 Impact factor: 3.490

4. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.

Authors: D B Clewell; D R Helinski
Journal: Proc Natl Acad Sci U S A Date: 1969-04 Impact factor: 11.205

5. Two replication initiation sites on R-plasmid DNA.

Authors: J H Crosa; L K Luttropp; F Heffron; S Falkow
Journal: Mol Gen Genet Date: 1975-09-15

6. Mutations in R factors of Escherichia coli causing an increased number of R-factor copies per chromosome.

Authors: K Nordström; L C Ingram; A Lundbäck
Journal: J Bacteriol Date: 1972-05 Impact factor: 3.490

7. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.

Authors: S N Cohen; A C Chang; L Hsu
Journal: Proc Natl Acad Sci U S A Date: 1972-08 Impact factor: 11.205

8. Circular R-factor molecules controlling penicillinase synthesis, replicating in Escherichia coli under either relaxed or stringent control.

Authors: P Kontomichalou; M Mitani; R C Clowes
Journal: J Bacteriol Date: 1970-10 Impact factor: 3.490

9. Genetic transformation in Escherichia coli K12.

Authors: S D Cosloy; M Oishi
Journal: Proc Natl Acad Sci U S A Date: 1973-01 Impact factor: 11.205

10. R factor proteins synthesized in Escherichia coli minicells: incorporation studies with different R factors and detection of deoxyribonucleic acid-binding proteins.

Authors: S B Levy
Journal: J Bacteriol Date: 1974-12 Impact factor: 3.490

4 in total