| Literature DB >> 35704558 |
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Abstract
[This corrects the article DOI: 10.1371/journal.pntd.0007552.].Entities:
Year: 2022 PMID: 35704558 PMCID: PMC9200301 DOI: 10.1371/journal.pntd.0010552
Source DB: PubMed Journal: PLoS Negl Trop Dis ISSN: 1935-2727
Pairwise FST values between 9 sampling locations.
| Barrio Florida | Aucayo | Indiana/Mazan | Nauta | Tamshiaco | Iquitos A | Iquitos B | Iquitos C | Iquitos D | |
|---|---|---|---|---|---|---|---|---|---|
|
| 0 | ||||||||
|
| 0.0793 | 0 | |||||||
|
| 0.03882 | 0.03062 | 0 | ||||||
|
| 0.03857 | 0.04908 | 0.05776 | 0 | |||||
|
| 0.05879 | 0.04171 | 0.05084 | 0.02182 | 0 | ||||
|
| 0.09909 | 0.04595 | 0.05694 | 0.06006 | 0.03617 | 0 | |||
|
| 0.03452 | 0.0221 | 0.01617 | 0.04593 | 0.01277 | 0.03446 | 0 | ||
|
| 0.11186 | 0.05321 | 0.08134 | 0.07741 | 0.02794 | 0.05779 | 0.0453 | 0 | |
|
| 0.03007 | 0.01994 | 0.01682 | 0.02788 | 0.00845 | 0.03074 | 0.00362 | 0.04614 | 0 |
* FST values statistically different from zero, indicating genetic differentiation (p < 0.05).
The fixation index, FST, ranges from 0 to 1 and measures the degree of genetic relatedness between two pairs of populations by comparing the variation observed in the subpopulation with the variation observed in the total population. Values approaching 0 represent panmixia, whereas values approaching 1 represent complete genetic isolation (non-interbreeding populations). Our findings show low to moderate genetic differentiation, with the greatest degree of differentiation observed for Barrio Florida (highest FST values overall) and a lower degree of differentiation for Iquitos sites (lower FST values).
Analysis of Molecular Variance (AMOVA) of Ae. aegypti mosquitoes using 8 microsatellite loci.
| Source of Variation | df | Sum of Squares | Variance components | Variation (%) | F-Statistic | P-value |
|---|---|---|---|---|---|---|
| Among groups | 5 | 40.895 | -0.00770 | -0.38% | FCT = 0.28534 | 0.61693 |
| Among populations within groups | 3 | 22.246 | 0.08117 | 4.05% | FSC = 0.04035 | <0.0001 |
| Among individuals within populations | 330 | 801.514 | 0.49834 | 24.87% | FIS = 0.25814 | <0.0001 |
| Within individuals | 339 | 485.500 | 1.43215 | 71.47% | FIT = 0.28534 | <0.0001 |
|
| 667 | 1350.155 | 2.00397 |
FCT, differentiation among groups.
FSC, differentiation among populations within groups (Iquitos samples).
FIS, differentiation among individuals within populations.
FIT, differentiation within individuals.
*Statistically significant values (p < 0.05).
AMOVA results showed that 71.5% of the total variation observed was attributable to differences within individuals; only 4.1% of the total variation was attributable to differences among samples collected within Iquitos, indicating a lack of genetic structure within the city.