Goodness-of-fit tests for locus order in three-point mapping.

For the case of three-point linkage analysis, a simple goodness-of-fit test is proposed to test the order of gene loci. To use it for testing one locus order against another, one may apply the principle that evidence for one order comes from lack of fit of the observations to the other order. With n = 30 offspring from a phase known triple backcross mating, at a recombination fraction of 20% between adjacent loci, the test achieves a power of 80% (5% significance level). Interference (real interference as well as interference assumed in the analysis) increases power.