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Literature DB >> 35697782

Illuminating RNA biology through imaging.

Phuong Le^1,2,3, Noorsher Ahmed^1,2,3,4, Gene W Yeo^5,6,7,8.

Abstract

RNA processing plays a central role in accurately transmitting genetic information into functional RNA and protein regulators. To fully appreciate the RNA life-cycle, tools to observe RNA with high spatial and temporal resolution are critical. Here we review recent advances in RNA imaging and highlight how they will propel the field of RNA biology. We discuss current trends in RNA imaging and their potential to elucidate unanswered questions in RNA biology.

Entities: Chemical

Mesh：

Substances：
RNA, Untranslated
RNA

Year: 2022 PMID： 35697782 DOI： 10.1038/s41556-022-00933-9

Source DB: PubMed Journal: Nat Cell Biol ISSN： 1465-7392 Impact factor: 28.213

139 in total

1. Single-cell gene expression profiling.

Authors: Jeffrey M Levsky; Shailesh M Shenoy; Rossanna C Pezo; Robert H Singer
Journal: Science Date: 2002-08-02 Impact factor: 47.728

2. In situ detection and genotyping of individual mRNA molecules.

Authors: Chatarina Larsson; Ida Grundberg; Ola Söderberg; Mats Nilsson
Journal: Nat Methods Date: 2010-04-11 Impact factor: 28.547

3. In situ genotyping individual DNA molecules by target-primed rolling-circle amplification of padlock probes.

Authors: Chatarina Larsson; Jørn Koch; Anders Nygren; George Janssen; Anton K Raap; Ulf Landegren; Mats Nilsson
Journal: Nat Methods Date: 2004-11-18 Impact factor: 28.547

4. Visualization of single RNA transcripts in situ.

Authors: A M Femino; F S Fay; K Fogarty; R H Singer
Journal: Science Date: 1998-04-24 Impact factor: 47.728

5. Programmable in situ amplification for multiplexed imaging of mRNA expression.

Authors: Harry M T Choi; Joann Y Chang; Le A Trinh; Jennifer E Padilla; Scott E Fraser; Niles A Pierce
Journal: Nat Biotechnol Date: 2010-10-31 Impact factor: 54.908

6. Imaging individual mRNA molecules using multiple singly labeled probes.

Authors: Arjun Raj; Patrick van den Bogaard; Scott A Rifkin; Alexander van Oudenaarden; Sanjay Tyagi
Journal: Nat Methods Date: 2008-09-21 Impact factor: 28.547

7. smiFISH and FISH-quant - a flexible single RNA detection approach with super-resolution capability.

Authors: Nikolay Tsanov; Aubin Samacoits; Racha Chouaib; Abdel-Meneem Traboulsi; Thierry Gostan; Christian Weber; Christophe Zimmer; Kazem Zibara; Thomas Walter; Marion Peter; Edouard Bertrand; Florian Mueller
Journal: Nucleic Acids Res Date: 2016-09-05 Impact factor: 16.971

Illuminating RNA biology through imaging.

1. Single-cell gene expression profiling.

2. In situ detection and genotyping of individual mRNA molecules.

3. In situ genotyping individual DNA molecules by target-primed rolling-circle amplification of padlock probes.

4. Visualization of single RNA transcripts in situ.

5. Programmable in situ amplification for multiplexed imaging of mRNA expression.

6. Imaging individual mRNA molecules using multiple singly labeled probes.

7. smiFISH and FISH-quant - a flexible single RNA detection approach with super-resolution capability.

8. Visualizing adenosine-to-inosine RNA editing in single mammalian cells.

9. SABER amplifies FISH: enhanced multiplexed imaging of RNA and DNA in cells and tissues.

10. Visualizing SNVs to quantify allele-specific expression in single cells.

Review 1. Aging RNA granule dynamics in neurodegeneration.