Literature DB >> 35696031

Microinjection of Zygotes for CRISPR/Cas9-Mediated Insertion of Transgenes into the Murine Rosa26 Safe Harbor.

Fabien Delerue1, Lars M Ittner2.   

Abstract

Genetically modified (GM) mice are widely used in biomedical research because they can address complex questions in an in-vivo setting that could not otherwise be addressed in-vitro. Microinjection of zygotes remains the most common technique to generate GM animals to date. Here, we describe the targeted insertion (knock-in) of transgenes by microinjection of 1-cell or 2-cell stage embryos into the murine Rosa26 safe harbor.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Genotyping; Microinjection; Pronuclei; Reimplantation; Rosa26; Transgene; Zygotes

Mesh：

Substances：

Year:  2022        PMID： 35696031     DOI： 10.1007/978-1-0716-2301-5_7

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  17 in total

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Journal:  Proc Natl Acad Sci U S A       Date:  1997-04-15       Impact factor: 11.205

Review 2.  Position-effect variegation and the new biology of heterochromatin.

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Journal:  Curr Opin Genet Dev       Date:  1994-04       Impact factor: 5.578

3.  Tild-CRISPR Allows for Efficient and Precise Gene Knockin in Mouse and Human Cells.

Authors:  Xuan Yao; Meiling Zhang; Xing Wang; Wenqin Ying; Xinde Hu; Pengfei Dai; Feilong Meng; Linyu Shi; Yun Sun; Ning Yao; Wanxia Zhong; Yun Li; Keliang Wu; Weiping Li; Zi-Jiang Chen; Hui Yang
Journal:  Dev Cell       Date:  2018-05-21       Impact factor: 12.270

4.  Site-specific integrase-mediated transgenesis in mice via pronuclear injection.

Authors:  Bosiljka Tasic; Simon Hippenmeyer; Charlene Wang; Matthew Gamboa; Hui Zong; Yanru Chen-Tsai; Liqun Luo
Journal:  Proc Natl Acad Sci U S A       Date:  2011-04-04       Impact factor: 11.205

5.  Purification of plasmid and BAC transgenic DNA constructs.

Authors:  Chengyu Liu; Yubin Du; Wen Xie; Changyun Gui
Journal:  Methods Mol Biol       Date:  2013

6.  Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease.

Authors:  Wenning Qin; Stephanie L Dion; Peter M Kutny; Yingfan Zhang; Albert W Cheng; Nathaniel L Jillette; Ankit Malhotra; Aron M Geurts; Yi-Guang Chen; Haoyi Wang
Journal:  Genetics       Date:  2015-03-27       Impact factor: 4.562

7.  Mouse Genome Database (MGD)-2017: community knowledge resource for the laboratory mouse.

Authors:  Judith A Blake; Janan T Eppig; James A Kadin; Joel E Richardson; Cynthia L Smith; Carol J Bult
Journal:  Nucleic Acids Res       Date:  2016-11-28       Impact factor: 16.971

8.  A targeted mutation at the known collagenase cleavage site in mouse type I collagen impairs tissue remodeling.

Authors:  X Liu; H Wu; M Byrne; J Jeffrey; S Krane; R Jaenisch
Journal:  J Cell Biol       Date:  1995-07       Impact factor: 10.539

9.  An inducible and reversible mouse genetic rescue system.

Authors:  Hongkui Zeng; Kyoji Horie; Linda Madisen; Maria N Pavlova; Galina Gragerova; Alex D Rohde; Brian A Schimpf; Yuqiong Liang; Ethan Ojala; Farah Kramer; Patricia Roth; Olga Slobodskaya; Io Dolka; Eileen A Southon; Lino Tessarollo; Karin E Bornfeldt; Alexander Gragerov; George N Pavlakis; George A Gaitanaris
Journal:  PLoS Genet       Date:  2008-05-09       Impact factor: 5.917

10.  Simple Genome Editing of Rodent Intact Embryos by Electroporation.

Authors:  Takehito Kaneko; Tomoji Mashimo
Journal:  PLoS One       Date:  2015-11-10       Impact factor: 3.240
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