Xiaohong Yu1, Jing Wang1, Qianqian Han1, Wen Chu1, Shaowen Lu1, Yu Liu2, Yi Peng1, Jie Xu1, Yanqing Shui1. 1. Department of Periodontology, School and Hospital of Stomatology, Kunming Medical University, Yunnan, People's Republic of China. 2. Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Kunming Medical University, Yunnan, People's Republic of China.
Abstract
Background: Yunnan Baiyao, as a traditional Chinese herbal remedy with definite curative effect, has recently been proven can promote the proliferation of osteoblasts and differentiation of human periodontal ligament fibroblasts. Bio-Oss® scaffold is a porous bone graft material of natural and antigen-free bovine bone origin. Methods: To observe the effect of Yunnan Baiyao on the differentiation of HPDLFs on the Bio-Oss® collagen scaffold in vivo, the HPDLFs-Bio-Oss® collagen complex was constructed in vitro, and Yunnan Baiyao aqueous solution was added, respectively. The complex was divided into Yunnan Baiyao group I (50 μg/mL), Yunnan Baiyao group II (100 μg/mL), positive control (rhBMP-2) group and sham group. HPDLFs were identified by immunocytochemistry and immunofluorescence. The compatibility of HPDLFs with Bio-Oss® collagen was observed by fluorescence microscope and scanning electron microscope. The specimens were taken for HE staining after 8 weeks since the complex was implanted into nude mice, and the expressions of osteocalcin (OC), bone sialoprotein (BSP), osteopontin (OPN), and collagen I (CON-I) were detected by immunohistochemistry and qRT-PCR. Results: The number of capillaries and osteoblasts increased significantly after Yunnan Baiyao stimulation, and the expressions of BSP, OC, OPN and CON-I were increased after Yunnan Baiyao stimulation in a dose-dependent manner. Conclusion: Yunnan Baiyao solution can promote the differentiation of HPDLFs and the generation of capillaries on Bio-Oss® collagen scaffold in a dose-dependent manner.
Background: Yunnan Baiyao, as a traditional Chinese herbal remedy with definite curative effect, has recently been proven can promote the proliferation of osteoblasts and differentiation of human periodontal ligament fibroblasts. Bio-Oss® scaffold is a porous bone graft material of natural and antigen-free bovine bone origin. Methods: To observe the effect of Yunnan Baiyao on the differentiation of HPDLFs on the Bio-Oss® collagen scaffold in vivo, the HPDLFs-Bio-Oss® collagen complex was constructed in vitro, and Yunnan Baiyao aqueous solution was added, respectively. The complex was divided into Yunnan Baiyao group I (50 μg/mL), Yunnan Baiyao group II (100 μg/mL), positive control (rhBMP-2) group and sham group. HPDLFs were identified by immunocytochemistry and immunofluorescence. The compatibility of HPDLFs with Bio-Oss® collagen was observed by fluorescence microscope and scanning electron microscope. The specimens were taken for HE staining after 8 weeks since the complex was implanted into nude mice, and the expressions of osteocalcin (OC), bone sialoprotein (BSP), osteopontin (OPN), and collagen I (CON-I) were detected by immunohistochemistry and qRT-PCR. Results: The number of capillaries and osteoblasts increased significantly after Yunnan Baiyao stimulation, and the expressions of BSP, OC, OPN and CON-I were increased after Yunnan Baiyao stimulation in a dose-dependent manner. Conclusion: Yunnan Baiyao solution can promote the differentiation of HPDLFs and the generation of capillaries on Bio-Oss® collagen scaffold in a dose-dependent manner.