| Literature DB >> 35674901 |
Laura Márquez-Expósito1,2, Carolina Lavoz3, Elena Cantero-Navarro1,2, Raúl R Rodrigues-Diez1,2, Sergio Mezzano3, Marta Ruiz-Ortega4,5.
Abstract
The NOTCH signaling pathway is an evolutionarily conserved family of transmembrane receptors, ligands, and transcription factors. The NOTCH signaling is activated in many biological processes including nephrogenesis, tubulogenesis, and glomerulogenesis, as well as during pathological situations. Activation of Notch signaling is characterized by successive proteolytic cleavages triggered by the interaction between membrane-bound Notch receptors and ligands expressed on neighboring cells. In chronic kidney diseases, activation of the canonical NOTCH signaling pathway has been described. The following protocols will allow the direct assessment of Jagged-1/NOTCH signaling activation in biopsies of patients with chronic kidney diseases and in murine experimental models of renal damage.Entities:
Keywords: Immunohistochemistry; In situ hybridization; Jagged-1; Kidney biopsies; NOTCH
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Year: 2022 PMID： 35674901 DOI： 10.1007/978-1-0716-2201-8_15
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745