Literature DB >> 3567312

In vitro calibration of the equilibrium reactions of the metallochromic indicator dye antipyrylazo III with calcium.

S Hollingworth, R W Aldrich, S M Baylor.   

Abstract

The equilibrium reactions of the metallochromic indicator dye Antipyrylazo III with calcium at physiological ionic strength have been investigated spectrophotometrically. Dye absorbance as a function of wavelength was measured at various total dye and calcium concentrations. Analysis of the absorbance spectra indicated that at pH 6.9 at least three calcium:dye complexes form, with 1:1, 1:2, and possibly 2:2 stoichiometries. The dissociation constant and the changes in dye extinction coefficients on formation of the 1:2 complex, the main complex which forms when Antipyrylazo III is used to study cytoplasmic calcium transients, have been characterized.

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Year:  1987        PMID： 3567312      PMCID： PMC1329904          DOI： 10.1016/S0006-3495(87)83360-X

Source DB:  PubMed          Journal:  Biophys J        ISSN： 0006-3495            Impact factor:   4.033


  19 in total

1.  Stoichiometries of arsenazo III-Ca complexes.

Authors:  P Palade; J Vergara
Journal:  Biophys J       Date:  1983-09       Impact factor: 4.033

2.  Measurement and modification of free calcium transients in frog skeletal muscle fibres by a metallochromic indicator dye.

Authors:  L Kovacs; E Rios; M F Schneider
Journal:  J Physiol       Date:  1983-10       Impact factor: 5.182

3.  Time course of calcium release and removal in skeletal muscle fibers.

Authors:  W Melzer; E Rios; M F Schneider
Journal:  Biophys J       Date:  1984-03       Impact factor: 4.033

4.  Use of metallochromic dyes to measure changes in myoplasmic calcium during activity in frog skeletal muscle fibres.

Authors:  S M Baylor; W K Chandler; M W Marshall
Journal:  J Physiol       Date:  1982-10       Impact factor: 5.182

5.  The binding of arsenazo III to cell components.

Authors:  T J Beeler; A Schibeci; A Martonosi
Journal:  Biochim Biophys Acta       Date:  1980-05-07

6.  Calcium transients and intramembrane charge movement in skeletal muscle fibres.

Authors:  L Kovács; E Ríos; M F Schneider
Journal:  Nature       Date:  1979-05-31       Impact factor: 49.962

7.  Optical measurements of intracellular pH and magnesium in frog skeletal muscle fibres.

Authors:  S M Baylor; W K Chandler; M W Marshall
Journal:  J Physiol       Date:  1982-10       Impact factor: 5.182

8.  Metal ion/buffer interactions. Stability of binary and ternary complexes containing 2-amino-2(hydroxymethyl)-1,3-propanediol (Tris) and adenosine 5'-triphosphate (ATP).

Authors:  B E Fischer; U K Häring; R Tribolet; H Sigel
Journal:  Eur J Biochem       Date:  1979-03

9.  Stoichiometry of the reactions of calcium with the metallochromic indicator dyes antipyrylazo III and arsenazo III.

Authors:  E Ríos; M F Schneider
Journal:  Biophys J       Date:  1981-12       Impact factor: 4.033

10.  Arsenazo III and antipyrylazo III calcium transients in single skeletal muscle fibers.

Authors:  P Palade; J Vergara
Journal:  J Gen Physiol       Date:  1982-04       Impact factor: 4.086
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  9 in total

1.  Fura-2 calcium transients in frog skeletal muscle fibres.

Authors:  S M Baylor; S Hollingworth
Journal:  J Physiol       Date:  1988-09       Impact factor: 5.182

2.  Simultaneous recording of calcium transients in skeletal muscle using high- and low-affinity calcium indicators.

Authors:  M G Klein; B J Simon; G Szucs; M F Schneider
Journal:  Biophys J       Date:  1988-06       Impact factor: 4.033

3.  Myoplasmic binding of fura-2 investigated by steady-state fluorescence and absorbance measurements.

Authors:  M Konishi; A Olson; S Hollingworth; S M Baylor
Journal:  Biophys J       Date:  1988-12       Impact factor: 4.033

4.  Excitation-contraction coupling in intact frog skeletal muscle fibers injected with mmolar concentrations of fura-2.

Authors:  S Hollingworth; A B Harkins; N Kurebayashi; M Konishi; S M Baylor
Journal:  Biophys J       Date:  1992-07       Impact factor: 4.033

5.  Calcium-induced inactivation of calcium release from the sarcoplasmic reticulum of skeletal muscle.

Authors:  W M Kwok; P M Best
Journal:  Pflugers Arch       Date:  1991-09       Impact factor: 3.657

6.  Simultaneous monitoring of changes in magnesium and calcium concentrations in frog cut twitch fibers containing antipyrylazo III.

Authors:  M Irving; J Maylie; N L Sizto; W K Chandler
Journal:  J Gen Physiol       Date:  1989-04       Impact factor: 4.086

7.  Myoplasmic calcium transients in intact frog skeletal muscle fibers monitored with the fluorescent indicator furaptra.

Authors:  M Konishi; S Hollingworth; A B Harkins; S M Baylor
Journal:  J Gen Physiol       Date:  1991-02       Impact factor: 4.086

8.  Myoplasmic calcium transients monitored with purpurate indicator dyes injected into intact frog skeletal muscle fibers.

Authors:  M Konishi; S M Baylor
Journal:  J Gen Physiol       Date:  1991-02       Impact factor: 4.086

9.  Physiological role and selectivity of the in situ potassium channel of the sarcoplasmic reticulum in skinned frog skeletal muscle fibers.

Authors:  C W Abramcheck; P M Best
Journal:  J Gen Physiol       Date:  1989-01       Impact factor: 4.086
  9 in total

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