| Literature DB >> 35668049 |
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Abstract
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Year: 2022 PMID: 35668049 PMCID: PMC9170811 DOI: 10.1111/jcmm.17329
Source DB: PubMed Journal: J Cell Mol Med ISSN: 1582-1838 Impact factor: 5.295
FIGURE 2Effects of H2 inhalation on cardiac function and myocardial structure changes in rats. (A) Survival curve of rats of each experimental group; (B) the plasma BNP concentration of each group, n = 5 per group; (C) images illustrating echocardiography of rat's heart; (D–G) EF (%); FS (%); LVIDd and LVIDs (n = 5 per group); (H) TEM images illustrating the cardiomyocytes (×30k magnification, scale bar 100 nm), the arrow indicates the mitochondria of cardiomyocytes; (I) the heart MDA concentration of each group, n = 5 per group; (J) the heart relative ·OH concentration of each group, n = 5 per group. Data are shown by mean ± SEM, ***p < 0.001 vs Sham group. #p < 0.05 ##p < 0.01 ###p < 0.001 vs MI group. &p < 0.05 vs MI + MCC950 group. $$$p < 0.001 vs MI + MCC950 group
FIGURE 3Effects of inhalation of H2 on myocardial fibrosis and fibrosis‐related proteins in MI rats. (A) The representative Masson images of rat whole heart and left ventricle; (B) fibrosis area of whole heart sections. n = 5 per group; (C) collagen volume fraction of left ventricle. n = 5 per group; (D) the representative immunohistochemical staining images of α‐SMA and fibronectin expression in each group (×20 magnification, scale bar 50 μm); (E–F) quantification of α‐SMA and fibronectin‐positive cells statistical chart, n = 5 per group; (G) the representative Western blot bands of TGF‐β, α‐SMA, Col I and Col III; (H–K) relative TGF‐β, α‐SMA, Col I and Col III protein level, n = 5. Data are shown by mean ± SEM, ***p < 0.001 vs Sham group. #p < 0.05 ##p < 0.01 ###p < 0.001 vs MI group. &p < 0.05 vs MI +MCC950 group