Literature DB >> 3565757

Detection and characterization of lipid hydroperoxides at picomole levels by high-performance liquid chromatography.

Y Yamamoto, M H Brodsky, J C Baker, B N Ames.   

Abstract

A new method for the detection of various lipid hydroperoxides and hydrogen peroxide at the picomole level has been developed by combining an HPLC system with an ultrasensitive analytical system based on the detection of chemiluminescence emitted by isoluminol in the presence of hydroperoxide and microperoxidase. This HPLC separation removes interfering antioxidants so that the method can be applied to biological samples such as blood plasma lipids. Several HPLC conditions are described which allow simple identification of different lipid hydroperoxides.

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Year:  1987        PMID： 3565757     DOI： 10.1016/0003-2697(87)90606-3

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  46 in total

1.  Rapid reprogramming of haemoglobin structure-function exposes multiple dual-antimicrobial potencies.

Authors:  Ruijuan Du; Bow Ho; Jeak Ling Ding
Journal:  EMBO J       Date:  2009-12-17       Impact factor: 11.598

2.  Electrospray MS/MS reveals extensive and nonspecific oxidation of cholesterol esters in human peripheral vascular lesions.

Authors:  Patrick M Hutchins; Ernest E Moore; Robert C Murphy
Journal:  J Lipid Res       Date:  2011-08-31       Impact factor: 5.922

Review 3.  Review of progress in sterol oxidations: 1987-1995.

Authors:  L L Smith
Journal:  Lipids       Date:  1996-05       Impact factor: 1.880

4.  Chemiluminescent determination of cholesterol hydroperoxides in human erythrocyte membrane.

Authors:  J Adachi; M Asano; T Naito; Y Ueno; Y Tatsuno
Journal:  Lipids       Date:  1998-12       Impact factor: 1.880

5.  Characterization of lipid hydroperoxides generated by photodynamic treatment of leukemia cells.

Authors:  G J Bachowski; W Korytowski; A W Girotti
Journal:  Lipids       Date:  1994-07       Impact factor: 1.880

6.  Analysis of lipid hydroperoxides and long-chain conjugated keto acids by negative ion electrospray mass spectrometry.

Authors:  D K MacMillan; R C Murphy
Journal:  J Am Soc Mass Spectrom       Date:  1995-12       Impact factor: 3.109

7.  Inhibition by interferon-gamma of human mononuclear cell-mediated low density lipoprotein oxidation. Participation of tryptophan metabolism along the kynurenine pathway.

Authors:  S Christen; S R Thomas; B Garner; R Stocker
Journal:  J Clin Invest       Date:  1994-05       Impact factor: 14.808

8.  A simple assay for lipid hydroperoxides based on triphenylphosphine oxidation and high-performance liquid chromatography.

Authors:  T Nakamura; H Maeda
Journal:  Lipids       Date:  1991-09       Impact factor: 1.880

9.  Hydroperoxides of erythrocyte phospholipid molecular species formed by lipoxygenase correlate with alpha-tocopherol levels.

Authors:  P Therond; M Couturier; J F Demelier; F Lemonnier
Journal:  Lipids       Date:  1996-07       Impact factor: 1.880

10.  Oxidative mechanisms of toxicity of low-intensity near-UV light in Salmonella typhimurium.

Authors:  G F Kramer; B N Ames
Journal:  J Bacteriol       Date:  1987-05       Impact factor: 3.490
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