Jichan Nie1, Chenyan Zhao2, Antonio Simone Laganà3, Xishi Liu4, Sun-Wei Guo5. 1. Department of General Gynecology, Shanghai Obstetrics and Gynecology Hospital, Fudan University, Shanghai, People's Republic of China. 2. Department of Pathology, Shanghai Obstetrics and Gynecology Hospital, Fudan University, Shanghai, People's Republic of China. 3. Department of Health Promotion, Mother and Child Care, Internal Medicine and Medical Specialties (PROMISE), University of Palermo, Palermo, Italy. 4. Department of General Gynecology, Shanghai Obstetrics and Gynecology Hospital, Fudan University, Shanghai, People's Republic of China; Shanghai Key Laboratory of Female Reproductive Endocrine-Related Diseases, Fudan University, Shanghai, People's Republic of China. 5. Shanghai Key Laboratory of Female Reproductive Endocrine-Related Diseases, Fudan University, Shanghai, People's Republic of China; Research Institute, Shanghai Obstetrics and Gynecology Hospital, Fudan University, Shanghai, People's Republic of China. Electronic address: hoxa10@outlook.com.
Abstract
OBJECTIVE: To investigate whether lesional immunostaining of putative biomarkers of recurrence and the extent of lesional and cortical fibroses are correlated with the severity of dysmenorrhea and serum antimüllerian hormone (AMH) levels in women with ovarian endometriomas (OEs). DESIGN: Retrospective cohort study. SETTING: Academic hospital. PATIENT(S): A total of 313 women with histologically confirmed OEs were recruited. Their demographic and clinical information and data on their preoperative AMH levels were collected. Additionally, samples of their lesional tissues and ovarian cortex tissues adjacent to the OE lesions were procured for histologic and immunohistochemistry analyses. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): All OE tissue samples were stained for phosphorylated nuclear factor κB p65 subunit, progesterone receptor isoform B, Slit2, and α-smooth muscle actin. In addition, the extent of lesional and cortical fibroses was quantitated by Masson trichrome staining. We evaluated the relationship between the lesion size; laterality; extent of lesional and cortical fibroses, along with the putative markers of recurrence; and severity of dysmenorrhea and preoperative serum AMH levels in patients with OE. RESULT(S): We found that the extent of lesional fibrosis was positively correlated with the severity of dysmenorrhea but had no impact on the AMH levels. On the other hand, the extent of cortical fibrosis, along with age, was negatively correlated with the AMH levels. CONCLUSION(S): The correlation between lesional fibrosis and the severity of dysmenorrhea and between cortical fibrosis and the AMH levels would call an early intervention once OE is diagnosed or suspected to prevent further pain and diminished ovarian reserve.
OBJECTIVE: To investigate whether lesional immunostaining of putative biomarkers of recurrence and the extent of lesional and cortical fibroses are correlated with the severity of dysmenorrhea and serum antimüllerian hormone (AMH) levels in women with ovarian endometriomas (OEs). DESIGN: Retrospective cohort study. SETTING: Academic hospital. PATIENT(S): A total of 313 women with histologically confirmed OEs were recruited. Their demographic and clinical information and data on their preoperative AMH levels were collected. Additionally, samples of their lesional tissues and ovarian cortex tissues adjacent to the OE lesions were procured for histologic and immunohistochemistry analyses. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): All OE tissue samples were stained for phosphorylated nuclear factor κB p65 subunit, progesterone receptor isoform B, Slit2, and α-smooth muscle actin. In addition, the extent of lesional and cortical fibroses was quantitated by Masson trichrome staining. We evaluated the relationship between the lesion size; laterality; extent of lesional and cortical fibroses, along with the putative markers of recurrence; and severity of dysmenorrhea and preoperative serum AMH levels in patients with OE. RESULT(S): We found that the extent of lesional fibrosis was positively correlated with the severity of dysmenorrhea but had no impact on the AMH levels. On the other hand, the extent of cortical fibrosis, along with age, was negatively correlated with the AMH levels. CONCLUSION(S): The correlation between lesional fibrosis and the severity of dysmenorrhea and between cortical fibrosis and the AMH levels would call an early intervention once OE is diagnosed or suspected to prevent further pain and diminished ovarian reserve.
Authors: Ferdinando Antonio Gulino; Valentina Dilisi; Stella Capriglione; Francesco Cannone; Francesco Catania; Francesco Giuseppe Martire; Attilio Tuscano; Marianna Gulisano; Valentina D'Urso; Alessandra Di Stefano; Monia Caterina Cimino; Maurizio Filippini; Silvia Latella; Margaret Sammarini; Giulia Musmeci; Marco Antonio Palumbo Journal: Front Endocrinol (Lausanne) Date: 2022-08-31 Impact factor: 6.055