Literature DB >> 3563522

A small gold-conjugated antibody label: improved resolution for electron microscopy.

J F Hainfeld.   

Abstract

A general method has been developed to make the smallest gold-conjugated antibody label yet developed for electron microscopy. It should have wide application in domainal mapping of single molecules or in pinpointing specific molecules, sites, or sequences in supramolecular complexes. It permits electron microscopic visualization of single antigen-binding antibody fragments (Fab') by covalently linking an 11-atom gold cluster to a specific residue on each Fab' such that the antigenic specificity and capacity are preserved. The distance of the gold cluster from the antigen is a maximum of 5.0 nanometers when the undecagold-Fab' probe is used as an immunolabel.

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Year:  1987        PMID： 3563522     DOI： 10.1126/science.3563522

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  20 in total

Review 1.  Preparation and high-resolution microscopy of gold cluster labeled nucleic acid conjugates and nanodevices.

Authors:  Richard D Powell; James F Hainfeld
Journal:  Micron       Date:  2010-09-08       Impact factor: 2.251

2.  Correlative Light and Scanning Electron Microscopy for Observing the Three-Dimensional Ultrastructure of Membranous Cell Organelles in Relation to Their Molecular Components.

Authors:  Daisuke Koga; Satoshi Kusumi; Hiroki Bochimoto; Tsuyoshi Watanabe; Tatsuo Ushiki
Journal:  J Histochem Cytochem       Date:  2015-09-15       Impact factor: 2.479

Review 3.  FluoroNanogold: an important probe for correlative microscopy.

Authors:  Toshihiro Takizawa; Richard D Powell; James F Hainfeld; John M Robinson
Journal:  J Chem Biol       Date:  2015-08-25

4.  The dimensions of the protein import channels in the outer and inner mitochondrial membranes.

Authors:  M P Schwartz; A Matouschek
Journal:  Proc Natl Acad Sci U S A       Date:  1999-11-09       Impact factor: 11.205

5.  On the feasibility of visualizing ultrasmall gold labels in biological specimens by STEM tomography.

Authors:  A A Sousa; M A Aronova; Y C Kim; L M Dorward; G Zhang; R D Leapman
Journal:  J Struct Biol       Date:  2007-06-28       Impact factor: 2.867

6.  The EM structure of human DNA polymerase gamma reveals a localized contact between the catalytic and accessory subunits.

Authors:  Elena Yakubovskaya; Mark Lukin; Zhixin Chen; John Berriman; Joseph S Wall; Ryuji Kobayashi; Caroline Kisker; Daniel F Bogenhagen
Journal:  EMBO J       Date:  2007-08-30       Impact factor: 11.598

7.  Chain Conformations at the Surface of a Polydisperse Amphiphilic Comb Copolymer Film.

Authors:  William A Kuhlman; Elsa A Olivetti; Linda G Griffith; Anne M Mayes
Journal:  Macromolecules       Date:  2006-07-25       Impact factor: 5.985

8.  The location of the carboxy-terminal region of gamma chains in fibrinogen and fibrin D domains.

Authors:  M W Mosesson; K R Siebenlist; D A Meh; J S Wall; J F Hainfeld
Journal:  Proc Natl Acad Sci U S A       Date:  1998-09-01       Impact factor: 11.205

9.  In vitro formation of recycling vesicles from endosomes requires adaptor protein-1/clathrin and is regulated by rab4 and the connector rabaptin-5.

Authors:  Adriana Pagano; Pascal Crottet; Cristina Prescianotto-Baschong; Martin Spiess
Journal:  Mol Biol Cell       Date:  2004-08-25       Impact factor: 4.138

10.  High-resolution visualization by field emission scanning electron microscopy of Enterococcus faecalis surface proteins encoded by the pheromone-inducible conjugative plasmid pCF10.

Authors:  S B Olmsted; S L Erlandsen; G M Dunny; C L Wells
Journal:  J Bacteriol       Date:  1993-10       Impact factor: 3.490
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