Taojun Wang1, Iris Rijnaarts2,3,4, Gerben D A Hermes2, Nicole M de Roos3, Ben J M Witteman3,5, Nicole J W de Wit4, Coen Govers4,6, Hauke Smidt2, Erwin G Zoetendal2. 1. Laboratory of Microbiology, Wageningen University & Research, Wageningen, the Netherlands. taojun.wang@wur.nl. 2. Laboratory of Microbiology, Wageningen University & Research, Wageningen, the Netherlands. 3. Division of Human Nutrition and Health, Wageningen University & Research, Wageningen, the Netherlands. 4. Wageningen Food and Biobased Research, Wageningen University & Research, Wageningen, the Netherlands. 5. Department of Gastroenterology and Hepatology, Hospital Gelderse Vallei, Ede, the Netherlands. 6. Cell Biology & Immunology, Wageningen University & Research, Wageningen, the Netherlands.
Abstract
BACKGROUND: Irritable bowel syndrome (IBS) is the most prevalent functional bowel disorder, but its pathophysiology is still unknown. Although a microbial signature associated with IBS severity has been suggested, its association with IBS severity still remains largely unknown. AIMS: This study aims to assess longitudinal dynamics of fecal microbiota and short-chain fatty acids (SCFAs) in different IBS severity groups and study the association with stool pattern, diet, depression, anxiety, and quality of life (QoL). METHODS: A longitudinal study was performed, including n = 91 IBS patients and n = 28 matched controls. All participants collected fecal samples for microbiota composition and SCFA analysis and completed validated questionnaires regarding IBS severity, stool pattern, depression, anxiety, and IBS-QoL at two timepoints with four weeks in-between. Diet was assessed at the first timepoint. RESULTS: Over time, 36% of IBS patients changed in severity group, and 53% changed in predominant stool pattern. The largest proportion of microbiota variation was explained by the individual (R2 = 70.07%). Microbiota alpha diversity and composition, and SCFAs did not differ between IBS severity groups, nor between IBS and controls. Relative abundances of Bifidobacterium, Terrisporobacter, and Turicibacter consistently differed between IBS and controls, but not between IBS severity groups. Large dynamics over time were observed in the association of microbiota composition with questionnaire data where IBS symptom severity was associated at T1 but not at T2. CONCLUSIONS: Fecal microbiota and SCFA signatures were not consistently associated with IBS severity over time, indicating the importance of repeated sampling in IBS research.
BACKGROUND: Irritable bowel syndrome (IBS) is the most prevalent functional bowel disorder, but its pathophysiology is still unknown. Although a microbial signature associated with IBS severity has been suggested, its association with IBS severity still remains largely unknown. AIMS: This study aims to assess longitudinal dynamics of fecal microbiota and short-chain fatty acids (SCFAs) in different IBS severity groups and study the association with stool pattern, diet, depression, anxiety, and quality of life (QoL). METHODS: A longitudinal study was performed, including n = 91 IBS patients and n = 28 matched controls. All participants collected fecal samples for microbiota composition and SCFA analysis and completed validated questionnaires regarding IBS severity, stool pattern, depression, anxiety, and IBS-QoL at two timepoints with four weeks in-between. Diet was assessed at the first timepoint. RESULTS: Over time, 36% of IBS patients changed in severity group, and 53% changed in predominant stool pattern. The largest proportion of microbiota variation was explained by the individual (R2 = 70.07%). Microbiota alpha diversity and composition, and SCFAs did not differ between IBS severity groups, nor between IBS and controls. Relative abundances of Bifidobacterium, Terrisporobacter, and Turicibacter consistently differed between IBS and controls, but not between IBS severity groups. Large dynamics over time were observed in the association of microbiota composition with questionnaire data where IBS symptom severity was associated at T1 but not at T2. CONCLUSIONS: Fecal microbiota and SCFA signatures were not consistently associated with IBS severity over time, indicating the importance of repeated sampling in IBS research.
Authors: Rapat Pittayanon; Jennifer T Lau; Yuhong Yuan; Grigorios I Leontiadis; Frances Tse; Michael Surette; Paul Moayyedi Journal: Gastroenterology Date: 2019-03-30 Impact factor: 22.682
Authors: Pelin Yilmaz; Laura Wegener Parfrey; Pablo Yarza; Jan Gerken; Elmar Pruesse; Christian Quast; Timmy Schweer; Jörg Peplies; Wolfgang Ludwig; Frank Oliver Glöckner Journal: Nucleic Acids Res Date: 2013-11-28 Impact factor: 16.971
Authors: Gerben D A Hermes; Dorien Reijnders; Ruud S Kootte; Gijs H Goossens; Hauke Smidt; Max Nieuwdorp; Ellen E Blaak; Erwin G Zoetendal Journal: Sci Rep Date: 2020-05-05 Impact factor: 4.379