| Literature DB >> 35622331 |
Anne Eugster1, Magnolia L Bostick2,3, Nidhi Gupta2, Encarnita Mariotti-Ferrandiz4, Gloria Kraus1, Wenzhao Meng5, Cinque Soto6, Johannes Trück7, Ulrik Stervbo8, Eline T Luning Prak9.
Abstract
The development of high-throughput sequencing of adaptive immune receptor repertoires (AIRR-seq of IG and TR rearrangements) has provided a new frontier for in-depth analysis of the immune system. The last decade has witnessed an explosion in protocols, experimental methodologies, and computational tools. In this chapter, we discuss the major considerations in planning a successful AIRR-seq experiment together with basic strategies for controlling and evaluating the outcome of the experiment. Members of the AIRR Community have authored several chapters in this edition, which cover step-by-step instructions to successfully conduct, analyze, and share an AIRR-seq project.Entities:
Keywords: AIRR-seq; Antibody; Immune repertoire; Immunoglobulin; Next-generation sequencing; T-cell receptor; V(D)J recombination
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Year: 2022 PMID： 35622331 DOI： 10.1007/978-1-0716-2115-8_15
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745