| Literature DB >> 35622320 |
Vincent H J van der Velden1, Lorenz Bastian2, Monika Brüggemann2, Alina M Hartmann2, Nikos Darzentas2.
Abstract
Identification of immunoglobulin (IG) and T-cell receptor (TR) gene rearrangements in acute lymphoblastic leukemia (ALL) patients at initial presentation are crucial for monitoring of minimal residual disease (MRD) during subsequent follow-up and thereby for appropriate risk-group stratification. Here we describe how RNA-Seq data can be generated and subsequently analyzed with ARResT/Interrogate to identify possible MRD markers. In addition to the procedures, possible pitfalls will be discussed. Similar strategies can be employed for other lymphoid malignancies, such as lymphoma and myeloma.Entities:
Keywords: Acute lymphoblastic leukemia; Gene rearrangements; Immunoglobulin; Marker identification; Minimal residual disease; RNA-Seq; T-cell receptor; Whole exome sequencing; Whole genome sequencing
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Year: 2022 PMID： 35622320 DOI： 10.1007/978-1-0716-2115-8_4
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745