Literature DB >> 3562228

Apparent stimulation of calf thymus DNA polymerase alpha by ATP.

C K Tan, M J So, K M Downey, A G So.   

Abstract

The mechanism by which millimolar concentrations of ATP stimulate the activity and increase the processivity of calf thymus DNA polymerase alpha has been investigated with poly(dA)/oligo(dT) as template/primer to eliminate possible effects due to primer synthesis. The effect of ATP on the rate of DNA synthesis with this template/primer was found to be dependent upon whether or not the ATP was neutralized and the species of buffer used in the reaction. The present studies suggest that ATP stimulation of calf thymus DNA polymerase can be attributed to changes in the pH of the reaction mixture, a shift in the magnesium ion optimum, or both. Furthermore, effects of ATP on the processivity of DNA polymerase alpha could be mimicked by lowering the pH of the reaction mixture.

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Year:  1987        PMID： 3562228      PMCID： PMC340633          DOI： 10.1093/nar/15.5.2269

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  15 in total

1.  Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.

Authors:  T Maniatis; A Jeffrey; H van deSande
Journal:  Biochemistry       Date:  1975-08-26       Impact factor: 3.162

2.  Studies on the purification and properties of a 6.8-S DNA polymerase activity found in calf-thymus DNA polymerase-alpha fraction.

Authors:  I P Hesslewood; A M Holmes; W F Wakeling; I R Johnston
Journal:  Eur J Biochem       Date:  1978-03

3.  A new mammalian DNA polymerase with 3' to 5' exonuclease activity: DNA polymerase delta.

Authors:  J J Byrnes; K M Downey; V L Black; A G So
Journal:  Biochemistry       Date:  1976-06-29       Impact factor: 3.162

4.  DNA polymerase 3 star requires ATP to start synthesis on a primed DNA.

Authors:  W Wickner; A Kornberg
Journal:  Proc Natl Acad Sci U S A       Date:  1973-12       Impact factor: 11.205

5.  In vitro conversion of MVM parvovirus single-stranded DNA to the replicative form by DNA polymerase alpha from Ehrlich ascites tumour cells.

Authors:  E A Faust; C D Rankin
Journal:  Nucleic Acids Res       Date:  1982-07-24       Impact factor: 16.971

6.  The kinetics and processivity of nucleic acid polymerases.

Authors:  W R McClure; Y Chow
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

7.  Further studies on partially purified calf thymus DNA polymerase a.

Authors:  K McKune; A M Holmes
Journal:  Nucleic Acids Res       Date:  1979-07-25       Impact factor: 16.971

8.  Mechanism of DNA elongation catalyzed by Escherichia coli DNA polymerase III, dnaZ protein, and DNA elongation factors I and III.

Authors:  S Wickner
Journal:  Proc Natl Acad Sci U S A       Date:  1976-10       Impact factor: 11.205

9.  An auxiliary protein for DNA polymerase-delta from fetal calf thymus.

Authors:  C K Tan; C Castillo; A G So; K M Downey
Journal:  J Biol Chem       Date:  1986-09-15       Impact factor: 5.157

10.  ATP activation of DNA polymerase III holoenzyme of Escherichia coli. I. ATP-dependent formation of an initiation complex with a primed template.

Authors:  P M Burgers; A Kornberg
Journal:  J Biol Chem       Date:  1982-10-10       Impact factor: 5.157
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  2 in total

1.  Further biochemical characterization of wheat DNA primase: possible functional implication of copurification with DNA polymerase A.

Authors:  P Laquel; M Castroviejo; S Litvak
Journal:  Nucleic Acids Res       Date:  1990-08-25       Impact factor: 16.971

2.  Autoantibody to the proliferating cell nuclear antigen neutralizes the activity of the auxiliary protein for DNA polymerase delta.

Authors:  C K Tan; K Sullivan; X Y Li; E M Tan; K M Downey; A G So
Journal:  Nucleic Acids Res       Date:  1987-11-25       Impact factor: 16.971
  2 in total

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