Sen Li1,2, Shuya Yang1,2, Yu Zhang1,2, Kai Huang1,2, Ting Liang1,2, Yu Chen1,2, Yingjie Guan1,2, Ruizhi Shang1,2, Tong Guan1,2, Jiang Wu3, Yingwei Chen4, Xiao Guan5,6. 1. School of Health Science and Engineering, University of Shanghai for Science and Technology, 516, Jungong Road, Shanghai, 200093, China. 2. National Grain Industry (Urban Grain and Oil Security) Technology Innovation Center, Shanghai, 200093, China. 3. Department of Gastroenterology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, 1665 Kongjiang Road, Shanghai, 200092, China. 4. Department of Gastroenterology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, 1665 Kongjiang Road, Shanghai, 200092, China. chenyingwei@xinhuamed.com.cn. 5. School of Health Science and Engineering, University of Shanghai for Science and Technology, 516, Jungong Road, Shanghai, 200093, China. gnxo@163.com. 6. National Grain Industry (Urban Grain and Oil Security) Technology Innovation Center, Shanghai, 200093, China. gnxo@163.com.
Abstract
PURPOSE: Inflammatory bowel disease (IBD) is a multifactorial chronic disease of the gastrointestinal tract. Dietary intervention in the treatment of IBD has gradually attracted more attention. In this study, amino acid-balanced diets (AABD) based on grains were developed and their influences on the regulation of IBD were investigated. METHODS: Dextran sodium sulfate (DSS)-induced acute colitis mice model was employed to evaluate the effects of AABD. Pathological symptoms, intestinal inflammation, gut barrier proteins and gut microbiota were determined after AABD intake. RESULTS: It was shown that AABD alleviated the symptoms of colitis by reducing the histological scores of mice colon, suppressing the expression of pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α) and upregulating the expression of tight junction proteins. Analysis of gut microbiota revealed that AABD altered the structure of gut microbiota by decreasing the abundance and richness of harmful bacteria induced by DSS (Escherichia-Shigella, Parasutterella, etc.) and increasing that of beneficial bacteria (Akkermansia, etc.). Correlation analysis indicated that the alterations of pro-inflammatory factors were related with the change of microbiota, suggesting that the inhibitory effects of AABD on inflammation might be due to its regulation gut microbiota. CONCLUSION: The AABD could efficiently mitigate colitis, and this study indicated that AABD could be applied as a promising dietary regulation strategy of IBD.
PURPOSE: Inflammatory bowel disease (IBD) is a multifactorial chronic disease of the gastrointestinal tract. Dietary intervention in the treatment of IBD has gradually attracted more attention. In this study, amino acid-balanced diets (AABD) based on grains were developed and their influences on the regulation of IBD were investigated. METHODS: Dextran sodium sulfate (DSS)-induced acute colitis mice model was employed to evaluate the effects of AABD. Pathological symptoms, intestinal inflammation, gut barrier proteins and gut microbiota were determined after AABD intake. RESULTS: It was shown that AABD alleviated the symptoms of colitis by reducing the histological scores of mice colon, suppressing the expression of pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α) and upregulating the expression of tight junction proteins. Analysis of gut microbiota revealed that AABD altered the structure of gut microbiota by decreasing the abundance and richness of harmful bacteria induced by DSS (Escherichia-Shigella, Parasutterella, etc.) and increasing that of beneficial bacteria (Akkermansia, etc.). Correlation analysis indicated that the alterations of pro-inflammatory factors were related with the change of microbiota, suggesting that the inhibitory effects of AABD on inflammation might be due to its regulation gut microbiota. CONCLUSION: The AABD could efficiently mitigate colitis, and this study indicated that AABD could be applied as a promising dietary regulation strategy of IBD.
Authors: Isabel Andújar; M Carmen Recio; Rosa M Giner; Elena Cienfuegos-Jovellanos; Sara Laghi; Begoña Muguerza; José Luis Ríos Journal: J Agric Food Chem Date: 2011-05-31 Impact factor: 5.279
Authors: Vaios Svolos; Richard Hansen; Ben Nichols; Christopher Quince; Umer Z Ijaz; Rodanthi T Papadopoulou; Christine A Edwards; David Watson; Adel Alghamdi; Asker Brejnrod; Cecilia Ansalone; Hazel Duncan; Lisa Gervais; Rachel Tayler; Jonathan Salmond; Daniele Bolognini; Robert Klopfleisch; Daniel R Gaya; Simon Milling; Richard K Russell; Konstantinos Gerasimidis Journal: Gastroenterology Date: 2018-12-11 Impact factor: 22.682
Authors: Eric A Franzosa; Alexandra Sirota-Madi; Julian Avila-Pacheco; Nadine Fornelos; Henry J Haiser; Stefan Reinker; Tommi Vatanen; A Brantley Hall; Himel Mallick; Lauren J McIver; Jenny S Sauk; Robin G Wilson; Betsy W Stevens; Justin M Scott; Kerry Pierce; Amy A Deik; Kevin Bullock; Floris Imhann; Jeffrey A Porter; Alexandra Zhernakova; Jingyuan Fu; Rinse K Weersma; Cisca Wijmenga; Clary B Clish; Hera Vlamakis; Curtis Huttenhower; Ramnik J Xavier Journal: Nat Microbiol Date: 2018-12-10 Impact factor: 17.745