Literature DB >> 35618900

Engineered Heart Tissues for Contractile, Structural, and Transcriptional Assessment of Human Pluripotent Stem Cell-Derived Cardiomyocytes in a Three-Dimensional, Auxotonic Environment.

Samantha Bremner1,2, Alex J Goldstein1,3,4, Ty Higashi1,5, Nathan J Sniadecki6,7,8,9.   

Abstract

Three-dimensional, human engineered heart tissue promotes maturation of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) and provides a useful platform for in vitro cardiac development and disease modeling. This protocol describes the generation of fibrin-based engineered heart tissues (EHTs) containing hiPSC-CMs and human stromal cells. The platform makes use of racks of silicone posts that fit a standard 24-well dish. Stromal cells and hiPSC-CMs are cast in a fibrin hydrogel suspended between two silicone posts, forming an engineered tissue that generates synchronous contractions. The platform described herein is amenable to various measures of cardiac function including measurement of contractile force and calcium handling, as well as molecular biology assays and immunostaining.
© 2022. Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Cardiac tissue engineering; Cardiomyocytes; Engineered heart tissue; Fibrin; Induced pluripotent stem cells

Mesh：

Substances：

Year:  2022        PMID： 35618900     DOI： 10.1007/978-1-0716-2261-2_6

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  9 in total

Review 1.  Maturing human pluripotent stem cell-derived cardiomyocytes in human engineered cardiac tissues.

Authors:  Nicole T Feric; Milica Radisic
Journal:  Adv Drug Deliv Rev       Date:  2015-05-05       Impact factor: 15.470

2.  Microfabricated silicone elastomeric post arrays for measuring traction forces of adherent cells.

Authors:  Nathan J Sniadecki; Christopher S Chen
Journal:  Methods Cell Biol       Date:  2007       Impact factor: 1.441

Review 3.  Cardiomyocyte maturation: advances in knowledge and implications for regenerative medicine.

Authors:  Elaheh Karbassi; Aidan Fenix; Silvia Marchiano; Naoto Muraoka; Kenta Nakamura; Xiulan Yang; Charles E Murry
Journal:  Nat Rev Cardiol       Date:  2020-02-03       Impact factor: 32.419

4.  Cronos Titin Is Expressed in Human Cardiomyocytes and Necessary for Normal Sarcomere Function.

Authors:  Rebecca J Zaunbrecher; Ashley N Abel; Kevin Beussman; Andrea Leonard; Marion von Frieling-Salewsky; Paul A Fields; Lil Pabon; Hans Reinecke; Xiulan Yang; Jesse Macadangdang; Deok-Ho Kim; Wolfgang A Linke; Nathan J Sniadecki; Michael Regnier; Charles E Murry
Journal:  Circulation       Date:  2019-10-07       Impact factor: 29.690

Review 5.  Perspectives on the Use of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in Biomedical Research.

Authors:  Christine L Mummery
Journal:  Stem Cell Reports       Date:  2018-12-11       Impact factor: 7.765

6.  Bioengineering adult human heart tissue: How close are we?

Authors:  Richard J Mills; James E Hudson
Journal:  APL Bioeng       Date:  2019-03-14

7.  Cardiomyopathy phenotypes in human-induced pluripotent stem cell-derived cardiomyocytes-a systematic review.

Authors:  Thomas Eschenhagen; Lucie Carrier
Journal:  Pflugers Arch       Date:  2018-10-15       Impact factor: 3.657

8.  Chromatin compartment dynamics in a haploinsufficient model of cardiac laminopathy.

Authors:  Alessandro Bertero; Paul A Fields; Alec S T Smith; Andrea Leonard; Kevin Beussman; Nathan J Sniadecki; Deok-Ho Kim; Hung-Fat Tse; Lil Pabon; Jay Shendure; William S Noble; Charles E Murry
Journal:  J Cell Biol       Date:  2019-08-08       Impact factor: 10.539

9.  Afterload promotes maturation of human induced pluripotent stem cell derived cardiomyocytes in engineered heart tissues.

Authors:  Andrea Leonard; Alessandro Bertero; Joseph D Powers; Kevin M Beussman; Shiv Bhandari; Michael Regnier; Charles E Murry; Nathan J Sniadecki
Journal:  J Mol Cell Cardiol       Date:  2018-03-28       Impact factor: 5.000
  9 in total
  1 in total

1.  Full-length dystrophin deficiency leads to contractile and calcium transient defects in human engineered heart tissues.

Authors:  Samantha B Bremner; Christian J Mandrycky; Andrea Leonard; Ruby M Padgett; Alan R Levinson; Ethan S Rehn; J Manuel Pioner; Nathan J Sniadecki; David L Mack
Journal:  J Tissue Eng       Date:  2022-08-17       Impact factor: 7.940
  1 in total

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