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Literature DB >> 35616858

Production of Recombinant Glycoproteins in Nicotiana tabacum BY-2 Suspension Cells.

Abstract

This protocol describes a robust method to obtain transgenic Nicotiana tabacum BY-2 cells that produce glycoproteins of interest via Agrobacterium tumefaciens transformation. Compared to biolistics-based transformation, this procedure requires only standard laboratory equipment.

Entities: Chemical

Keywords: Agrobacterium tumefaciens; Glycoproteins; Nicotiana tabacum BY-2; Recombinant proteins; Transformation

Mesh：

Substances：

Year: 2022 PMID： 35616858 DOI： 10.1007/978-1-0716-2241-4_5

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

24 in total

Review 1. Large-scale production of pharmaceutical proteins in plant cell culture-the Protalix experience.

Authors: Yoram Tekoah; Avidor Shulman; Tali Kizhner; Ilya Ruderfer; Liat Fux; Yakir Nataf; Daniel Bartfeld; Tami Ariel; Svetlana Gingis-Velitski; Uri Hanania; Yoseph Shaaltiel
Journal: Plant Biotechnol J Date: 2015-06-23 Impact factor: 9.803

2. Purification and biochemical characterization of NpABCG5/NpPDR5, a plant pleiotropic drug resistance transporter expressed in Nicotiana tabacum BY-2 suspension cells.

Authors: Frédéric Toussaint; Baptiste Pierman; Aurélie Bertin; Daniel Lévy; Marc Boutry
Journal: Biochem J Date: 2017-05-04 Impact factor: 3.857

3. High efficiency transformation of cultured tobacco cells.

Authors: G An
Journal: Plant Physiol Date: 1985-10 Impact factor: 8.340

4. Mutational analysis of the transcriptional activator VirG of Agrobacterium tumefaciens.

Authors: E P Scheeren-Groot; K W Rodenburg; A den Dulk-Ras; S C Turk; P J Hooykaas
Journal: J Bacteriol Date: 1994-11 Impact factor: 3.490

5. Control of expression of Agrobacterium vir genes by synergistic actions of phenolic signal molecules and monosaccharides.

Authors: N Shimoda; A Toyoda-Yamamoto; J Nagamine; S Usami; M Katayama; Y Sakagami; Y Machida
Journal: Proc Natl Acad Sci U S A Date: 1990-09 Impact factor: 11.205

6. NtPDR1, a plasma membrane ABC transporter from Nicotiana tabacum, is involved in diterpene transport.

Authors: Jérôme Crouzet; Julien Roland; Emmanuel Peeters; Tomasz Trombik; Eric Ducos; Joseph Nader; Marc Boutry
Journal: Plant Mol Biol Date: 2013-04-07 Impact factor: 4.076

7. Activity of the purified plant ABC transporter NtPDR1 is stimulated by diterpenes and sesquiterpenes involved in constitutive and induced defenses.

Authors: Baptiste Pierman; Frédéric Toussaint; Aurélie Bertin; Daniel Lévy; Nicolas Smargiasso; Edwin De Pauw; Marc Boutry
Journal: J Biol Chem Date: 2017-09-29 Impact factor: 5.157

8. Expression of a constitutively activated plasma membrane H⁺-ATPase in Nicotiana tabacum BY-2 cells results in cell expansion.

Authors: Marta Niczyj; Antoine Champagne; Iftekhar Alam; Joseph Nader; Marc Boutry
Journal: Planta Date: 2016-07-21 Impact factor: 4.116