Literature DB >> 35612747

Proteomic Profiling of the Interplay Between a Bacterial Pathogen and Host Uncovers Novel Anti-Virulence Strategies.

Arjun Sukumaran1, Jennifer Geddes-McAlister2.   

Abstract

Bottom-up proteomics enables a systems-level analysis of proteins involved in a particular sample set. In this protocol, we describe the workflow to prepare Klebsiella pneumoniae and macrophage cells for co-culture, how to extract and prepare samples for analysis by high-resolution mass spectrometry, and lastly, how to analyze the output data files. This workflow allows for the identification of proteins involved in both the bacterial and host perspective during pathogenesis.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Bacterial pathogen; Macrophage; Mass spectrometry; Quantitative proteomics

Mesh：

Substances：

Year:  2022        PMID： 35612747     DOI： 10.1007/978-1-0716-2124-0_17

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  7 in total

Review 1.  Decoding communication patterns of the innate immune system by quantitative proteomics.

Authors:  Arjun Sukumaran; Jeremia M Coish; Jason Yeung; Benjamin Muselius; Mihaela Gadjeva; Adam J MacNeil; Jennifer Geddes-McAlister
Journal:  J Leukoc Biol       Date:  2019-09-26       Impact factor: 4.962

Review 2.  Klebsiella pneumoniae: Going on the Offense with a Strong Defense.

Authors:  Michelle K Paczosa; Joan Mecsas
Journal:  Microbiol Mol Biol Rev       Date:  2016-06-15       Impact factor: 11.056

3.  Risk factors for tracheobronchial acquisition of resistant Gram-negative bacterial pathogens in mechanically ventilated ICU patients.

Authors:  Ilias Papakonstantinou; Epameinondas Angelopoulos; Ioannis Baraboutis; Efstathia Perivolioti; Maria Parisi; Zoe Psaroudaki; Efstathia Kampisiouli; Athina Argyropoulou; Serafeim Nanas; Christina Routsi
Journal:  J Chemother       Date:  2014-07-01       Impact factor: 1.714

Review 4.  Complicated catheter-associated urinary tract infections due to Escherichia coli and Proteus mirabilis.

Authors:  S M Jacobsen; D J Stickler; H L T Mobley; M E Shirtliff
Journal:  Clin Microbiol Rev       Date:  2008-01       Impact factor: 26.132

5.  Host and microbiota factors that control Klebsiella pneumoniae mucosal colonization in mice.

Authors:  Helen Y Lau; Gary B Huffnagle; Thomas A Moore
Journal:  Microbes Infect       Date:  2008-08-13       Impact factor: 2.700

6.  Protocol for micro-purification, enrichment, pre-fractionation and storage of peptides for proteomics using StageTips.

Authors:  Juri Rappsilber; Matthias Mann; Yasushi Ishihama
Journal:  Nat Protoc       Date:  2007       Impact factor: 13.491

Review 7.  Fun(gi)omics: Advanced and Diverse Technologies to Explore Emerging Fungal Pathogens and Define Mechanisms of Antifungal Resistance.

Authors:  B Ball; M Langille; J Geddes-McAlister
Journal:  mBio       Date:  2020-10-06       Impact factor: 7.867
  7 in total

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