Literature DB >> 35612739

Phosphoproteomics and Organelle Proteomics in Pancreatic Islets.

Özüm Sehnaz Caliskan1,2, Giorgia Massacci3, Natalie Krahmer4,5, Francesca Sacco6.   

Abstract

Over the recent years, mass spectrometry (MS)-based proteomics has undergone dramatic advances in sample preparation, instrumentation, and computational methods. Here, we describe in detail, how a workflow quantifies global protein phosphorylation in pancreatic islets and characterizes intracellular organelle composition on protein level by MS-based proteomics.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Mass spectrometry-based phosphoproteomics; Organelles; Pancreatic islets; Protein correlation profiling

Mesh：

Year:  2022        PMID： 35612739     DOI： 10.1007/978-1-0716-2124-0_9

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  7 in total

1.  High-throughput phosphoproteomics reveals in vivo insulin signaling dynamics.

Authors:  Sean J Humphrey; S Babak Azimifar; Matthias Mann
Journal:  Nat Biotechnol       Date:  2015-08-17       Impact factor: 54.908

2.  Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling.

Authors:  Kirti Sharma; Rochelle C J D'Souza; Stefka Tyanova; Christoph Schaab; Jacek R Wiśniewski; Jürgen Cox; Matthias Mann
Journal:  Cell Rep       Date:  2014-08-21       Impact factor: 9.423

3.  Robust prediction of the MASCOT score for an improved quality assessment in mass spectrometric proteomics.

Authors:  Thomas Koenig; Bjoern H Menze; Marc Kirchner; Flavio Monigatti; Kenneth C Parker; Thomas Patterson; Judith Jebanathirajah Steen; Fred A Hamprecht; Hanno Steen
Journal:  J Proteome Res       Date:  2008-08-16       Impact factor: 4.466

4.  Phosphoproteomics Reveals the GSK3-PDX1 Axis as a Key Pathogenic Signaling Node in Diabetic Islets.

Authors:  Francesca Sacco; Anett Seelig; Sean J Humphrey; Natalie Krahmer; Francesco Volta; Alessio Reggio; Piero Marchetti; Jantje Gerdes; Matthias Mann
Journal:  Cell Metab       Date:  2019-03-14       Impact factor: 27.287

5.  Protocol for micro-purification, enrichment, pre-fractionation and storage of peptides for proteomics using StageTips.

Authors:  Juri Rappsilber; Matthias Mann; Yasushi Ishihama
Journal:  Nat Protoc       Date:  2007       Impact factor: 13.491

6.  Machine Learning-based Classification of Diffuse Large B-cell Lymphoma Patients by Their Protein Expression Profiles.

Authors:  Sally J Deeb; Stefka Tyanova; Michael Hummel; Marc Schmidt-Supprian; Juergen Cox; Matthias Mann
Journal:  Mol Cell Proteomics       Date:  2015-08-26       Impact factor: 5.911

7.  A practical guide to rodent islet isolation and assessment.

Authors:  Jeffrey D Carter; Stacey B Dula; Kathryn L Corbin; Runpei Wu; Craig S Nunemaker
Journal:  Biol Proced Online       Date:  2009-12-03       Impact factor: 3.244
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.