Literature DB >> 35612738

HUNTER: Sensitive Automated Characterization of Proteolytic Systems by N Termini Enrichment from Microscale Specimen.

Anuli C Uzozie1,2, Janice Tsui1,2, Philipp F Lange3,4,5.   

Abstract

Proteolysis occurs at low frequency in the cellular environment. Protein N termini reveal essential mechanisms associated with cellular functions, and are useful indicators to track dysfunctional regulation of proteins and pathways in diseases. N terminomics has so far relied on labor-intensive methods, which require relatively large starting sample amounts rendering it ill-suited for high-throughput systems biology studies. Here, we describe protocols for the first scalable and automatable method for sensitive enrichment and identification of N termini from minute samples.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  N termini; Proteoform; Proteolysis

Mesh：

Substances：

Year:  2022        PMID： 35612738     DOI： 10.1007/978-1-0716-2124-0_8

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  16 in total

Review 1.  N- and C-terminal degradomics: new approaches to reveal biological roles for plant proteases from substrate identification.

Authors:  Pitter F Huesgen; Christopher M Overall
Journal:  Physiol Plant       Date:  2011-12-07       Impact factor: 4.500

2.  Identifying and quantifying proteolytic events and the natural N terminome by terminal amine isotopic labeling of substrates.

Authors:  Oded Kleifeld; Alain Doucet; Anna Prudova; Ulrich auf dem Keller; Magda Gioia; Jayachandran N Kizhakkedathu; Christopher M Overall
Journal:  Nat Protoc       Date:  2011-09-22       Impact factor: 13.491

Review 3.  Statistical design of quantitative mass spectrometry-based proteomic experiments.

Authors:  Ann L Oberg; Olga Vitek
Journal:  J Proteome Res       Date:  2009-05       Impact factor: 4.466

Review 4.  Protein TAILS: when termini tell tales of proteolysis and function.

Authors:  Philipp F Lange; Christopher M Overall
Journal:  Curr Opin Chem Biol       Date:  2013-01-06       Impact factor: 8.822

Review 5.  Ensembles of protein termini and specific proteolytic signatures as candidate biomarkers of disease.

Authors:  Pitter F Huesgen; Philipp F Lange; Christopher M Overall
Journal:  Proteomics Clin Appl       Date:  2014-03-27       Impact factor: 3.494

Review 6.  Combinatorial degradomics: Precision tools to unveil proteolytic processes in biological systems.

Authors:  Simonas Savickas; Philipp Kastl; Ulrich Auf dem Keller
Journal:  Biochim Biophys Acta Proteins Proteom       Date:  2020-02-20       Impact factor: 3.036

7.  Measurement of protein using bicinchoninic acid.

Authors:  P K Smith; R I Krohn; G T Hermanson; A K Mallia; F H Gartner; M D Provenzano; E K Fujimoto; N M Goeke; B J Olson; D C Klenk
Journal:  Anal Biochem       Date:  1985-10       Impact factor: 3.365

8.  Sensitive Determination of Proteolytic Proteoforms in Limited Microscale Proteome Samples.

Authors:  Samuel S H Weng; Fatih Demir; Enes K Ergin; Sabrina Dirnberger; Anuli Uzozie; Domenic Tuscher; Lorenz Nierves; Janice Tsui; Pitter F Huesgen; Philipp F Lange
Journal:  Mol Cell Proteomics       Date:  2019-08-30       Impact factor: 5.911

9.  Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome.

Authors:  Philipp F Lange; Pitter F Huesgen; Karen Nguyen; Christopher M Overall
Journal:  J Proteome Res       Date:  2014-03-10       Impact factor: 4.466

10.  Importance of Block Randomization When Designing Proteomics Experiments.

Authors:  Bram Burger; Marc Vaudel; Harald Barsnes
Journal:  J Proteome Res       Date:  2020-10-05       Impact factor: 4.466
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