Literature DB >> 35612734

Rodent Lung Tissue Sample Preparation and Processing for Shotgun Proteomics.

Hadeesha Piyadasa1,2,3, Ying Lao1, Oleg Krokhin1, Neeloffer Mookherjee4,5.   

Abstract

Mass spectrometry (MS) is a routinely used approach to characterize global protein profile in various biological samples. Here we describe rodent lung tissue homogenization, sample preparation, and liquid chromatography with tandem mass spectrometry (LC-MS/MS) method for shotgun proteomics.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Mass spectrometry; Rodent lungs; Sample preparation; Shotgun proteomics

Mesh：

Year:  2022        PMID： 35612734     DOI： 10.1007/978-1-0716-2124-0_4

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  4 in total

1.  3D HPLC-MS with Reversed-Phase Separation Functionality in All Three Dimensions for Large-Scale Bottom-Up Proteomics and Peptide Retention Data Collection.

Authors:  Vic Spicer; Peyman Ezzati; Haley Neustaeter; Ronald C Beavis; John A Wilkins; Oleg V Krokhin
Journal:  Anal Chem       Date:  2016-02-18       Impact factor: 6.986

2.  TMT-based quantitative proteomics reveals suppression of SLC3A2 and ATP1A3 expression contributes to the inhibitory role of acupuncture on airway inflammation in an OVA-induced mouse asthma model.

Authors:  Weifeng Tang; Ming Dong; Fangzhou Teng; Jie Cui; Xueyi Zhu; Wenqian Wang; Tulake Wuniqiemu; Jingjing Qin; Shiyuan Wang; Jingcheng Dong; Ying Wei
Journal:  Biomed Pharmacother       Date:  2020-12-16       Impact factor: 6.529

3.  Interferon γ induced compositional changes in human bone marrow derived mesenchymal stem/stromal cells.

Authors:  Qingdong Guan; Peyman Ezzati; Victor Spicer; Oleg Krokhin; Donna Wall; John A Wilkins
Journal:  Clin Proteomics       Date:  2017-07-06       Impact factor: 3.988

4.  Integrating Proteomes for Lung Tissues and Lavage Reveals Pathways That Link Responses in Allergen-Challenged Mice.

Authors:  Thomas H Mahood; Christopher D Pascoe; Tobias K Karakach; Aruni Jha; Sujata Basu; Peyman Ezzati; Victor Spicer; Neeloffer Mookherjee; Andrew J Halayko
Journal:  ACS Omega       Date:  2021-01-05
  4 in total

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