Edmund Tsui1, Judy L Chen2, Nicholas J Jackson3, Omar Leyva2, Haroon Rasheed4, Elmira Baghdasaryan5, Simon S M Fung2, Deborah K McCurdy6, Srinivas R Sadda5, Gary N Holland2. 1. From the Ocular Inflammatory Disease Center, UCLA Stein Eye Institute (E.T., J.L.C., O.L., S.S.M.F., G.N.H.), Los Angeles; Department of Ophthalmology (E.T., J.L.C., O.L., H.R., E.B., S.S.M.F., S.R.S., G.N.H.). Electronic address: etsui@mednet.ucla.edu. 2. From the Ocular Inflammatory Disease Center, UCLA Stein Eye Institute (E.T., J.L.C., O.L., S.S.M.F., G.N.H.), Los Angeles; Department of Ophthalmology (E.T., J.L.C., O.L., H.R., E.B., S.S.M.F., S.R.S., G.N.H.). 3. Division of General Internal Medicine and Health Services Research, Department of Medicine (N.J.J.). 4. Doheny Eye Institute, Pasadena (E.B., S.R.S.), California, USA. 5. Department of Ophthalmology (E.T., J.L.C., O.L., H.R., E.B., S.S.M.F., S.R.S., G.N.H.); Division of General Internal Medicine and Health Services Research, Department of Medicine (N.J.J.). 6. Department of Pediatrics, David Geffen School of Medicine at UCLA, Los Angeles.
Abstract
PURPOSE: To evaluate the feasibility of anterior segment optical coherence tomography (AS-OCT) for measuring anterior chamber (AC) cells in children with uveitis and to compare different AS-OCT acquisition modes. DESIGN: Validity and reliability analysis. METHODS: We enrolled children younger than 18 years who had uveitis involving the anterior segment and children without eye disease as controls. All underwent clinical grading of AC cells. AC images of each eye were obtained using the Optovue Avanti RTVue XR AS-OCT. Two acquisition modes were used: a single cross-sectional line scan and an 8-line radial scan in an asterisk pattern. Two independent, masked graders counted cells manually on AS-OCT images. Rater agreement was assessed using intraclass correlation (ICC). RESULTS: Included were 30 children (59 eyes) with uveitis (median age 13.0 years, range 3-17 years) and 20 control children (40 eyes, median age 10.5 years, range 4-17 years). The number of eyes assigned each clinical grade of cells were as follows: none, 32 (54%); 0.5+, 12 (20.3%); 1+, 5 (8.5%); 2+, 8 (13.6%); 3+, 2 (3.4%). ICC of graders for line and radial scan protocols were 0.87 and 0.90. There was no significant difference between acquisition modes for pooled grader results (95% CI for difference: -0.04 to 0.14). ICC of cell counts between line and radial scan protocols was 0.85 (95% CI: 0.69-0.90). No control eyes had cells on AS-OCT images. CONCLUSIONS: Quantification of AC cell in children with uveitis is feasible with AS-OCT and has excellent reliability between different graders and acquisition modes.
PURPOSE: To evaluate the feasibility of anterior segment optical coherence tomography (AS-OCT) for measuring anterior chamber (AC) cells in children with uveitis and to compare different AS-OCT acquisition modes. DESIGN: Validity and reliability analysis. METHODS: We enrolled children younger than 18 years who had uveitis involving the anterior segment and children without eye disease as controls. All underwent clinical grading of AC cells. AC images of each eye were obtained using the Optovue Avanti RTVue XR AS-OCT. Two acquisition modes were used: a single cross-sectional line scan and an 8-line radial scan in an asterisk pattern. Two independent, masked graders counted cells manually on AS-OCT images. Rater agreement was assessed using intraclass correlation (ICC). RESULTS: Included were 30 children (59 eyes) with uveitis (median age 13.0 years, range 3-17 years) and 20 control children (40 eyes, median age 10.5 years, range 4-17 years). The number of eyes assigned each clinical grade of cells were as follows: none, 32 (54%); 0.5+, 12 (20.3%); 1+, 5 (8.5%); 2+, 8 (13.6%); 3+, 2 (3.4%). ICC of graders for line and radial scan protocols were 0.87 and 0.90. There was no significant difference between acquisition modes for pooled grader results (95% CI for difference: -0.04 to 0.14). ICC of cell counts between line and radial scan protocols was 0.85 (95% CI: 0.69-0.90). No control eyes had cells on AS-OCT images. CONCLUSIONS: Quantification of AC cell in children with uveitis is feasible with AS-OCT and has excellent reliability between different graders and acquisition modes.
Authors: Sumit Sharma; Careen Y Lowder; Amit Vasanji; Kimberly Baynes; Peter K Kaiser; Sunil K Srivastava Journal: Ophthalmology Date: 2015-04-04 Impact factor: 12.079