Literature DB >> 35604923

Correction: Expression from DIF1-motif promoters of hetR and patS is dependent on HetZ and modulated by PatU3 during heterocyst differentiation.

Yaru Du, He Zhang, Hong Wang, Shuai Wang, Qiqin Lei, Chao Li, Renqiu Kong, Xudong Xu.   

Abstract

[This corrects the article DOI: 10.1371/journal.pone.0232383.].

Entities:  

Year:  2022        PMID: 35604923      PMCID: PMC9126369          DOI: 10.1371/journal.pone.0269050

Source DB:  PubMed          Journal:  PLoS One        ISSN: 1932-6203            Impact factor:   3.752


In Fig 5, the bent arrow, which indicates the transcription start point, and ‘-272’ are positioned incorrectly in the sequence. The electrophoretogram of the 5′ RACE-PCR products of hetR transcripts in the wild type and the mutant is missing. Please see the correct Fig 5 here.
Fig 5

Differences between Anabaena 7120 and the P-DIF1- strain in heterocyst differentiation and expression of hetR.

(A) Photomicrographs of Anabaena 7120 and the P-DIF1- strain at 24 h and 48 h after nitrogen stepdown. Frequencies of heterocysts/proheterocysts are indicated. (B) A stretch of sequence upstream of hetR, including the DIF1 motif, potential HetR-binding sequence and the tsp at -272 (left), and the electrophoretogram of the 5′ RACE-PCR products of hetR transcripts in the two strains at 18 h after nitrogen stepdown (right). Each band of the RACE-PCR (indicated with tsp) was confirmed by sequencing.

Differences between Anabaena 7120 and the P-DIF1- strain in heterocyst differentiation and expression of hetR.

(A) Photomicrographs of Anabaena 7120 and the P-DIF1- strain at 24 h and 48 h after nitrogen stepdown. Frequencies of heterocysts/proheterocysts are indicated. (B) A stretch of sequence upstream of hetR, including the DIF1 motif, potential HetR-binding sequence and the tsp at -272 (left), and the electrophoretogram of the 5′ RACE-PCR products of hetR transcripts in the two strains at 18 h after nitrogen stepdown (right). Each band of the RACE-PCR (indicated with tsp) was confirmed by sequencing. Accordingly, the caption for Fig 5 has been updated to include updated information. Please see the complete, correct Fig 5 caption here. In the Discussion, there is an error in the first two sentences of the third paragraph. The correct sentences are: First, we showed that the DIF1-motif promoter is responsible for the upregulation of hetR in (pro)heterocysts. Substitutions at the DIF1 motif greatly reduced the transcription activity of P; a mutant of Anabaena 7120 with the DIF1 motif of hetR substituted in the genome showed no transcription from the tsp -272 (previously -271, the heterocyst-specific tsp in the wild type [47]).
  1 in total

1.  Expression from DIF1-motif promoters of hetR and patS is dependent on HetZ and modulated by PatU3 during heterocyst differentiation.

Authors:  Yaru Du; He Zhang; Hong Wang; Shuai Wang; Qiqin Lei; Chao Li; Renqiu Kong; Xudong Xu
Journal:  PLoS One       Date:  2020-07-23       Impact factor: 3.240

  1 in total

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