Occurrence of multiple drug-resistant Shiga toxigenic Escherichia coli in raw milk samples collected from retail outlets in South India.

The prevalence of Shiga-toxigenic E. coli (STEC), harbouring multidrug-resistant genes in raw milk collected from household vendors and cooperative milk marketing societies in Ernakulam District, Kerala, India, has been investigated. A higher prevalence of STEC pathotypes, with 47.16% (20 out of 42 samples), was observed in raw milk. A total of 157 STEC isolates were identified, which included notorious pathoserotypes, E. coli O157 group (10.19%) and E. coli non-O157 group 5.73%). All of the tested STEC isolates were multidrug-resistant and showed resistance to at least six different antibiotics. Two of the isolates showed resistance to 14 different antibiotics tested. Cent percentage resistance was observed for Penicillin, Cefalexine, Rifampicin, Methicillin, and Novobiocin. We observed in phenotypic assays that 26.75% of STEC isolates are ESBL producers. The bla TEM gene, a characteristic marker for ESBL production, was detected in 42.85% of the isolates. The study points out the risk of virulent and multidrug-resistant STEC in raw milk and the need for stringent quality surveillance and assurance plans to alleviate the potential public health threat. Supplementary Information: The online version contains supplementary material available at 10.1007/s13197-021-05226-x. © Association of Food Scientists & Technologists (India) 2021.