Present and pioneer methods of early detection of food borne pathogens.

Food-borne pathogens are a severe threat to human illness and death world-wide. Researchers have reported more than 250 food-borne diseases. Most of these are infections caused by a wide variety of bacteria, viruses, and parasites. It has a significant economic impact also. Detection of pathogenic microbes is thus essential for food safety. Such identification techniques could meet the following parameters viz., the accuracy of detection techniques that are quick, efficient, economical, highly sensitive, specific, and non-labor intensive. The various available methods for detecting food pathogens are classified into different groups, each having its advantages and disadvantages. The conventional methods are usually the first choice of detection even though they are laborious. Modern techniques such as biosensors, immunological assays, and macromolecule-based (nucleic acid) methods are being developed and refined to overcome traditional methods' limitations. Early detection of pathogens and secure food safety at each stage of food processing to storage, utilizing improved methodologies are mandatory. This review summarizes the deadly food pathogens leading to significant outbreaks and discusses the importance of early detection methods and advanced detection methods in comparison. © Association of Food Scientists & Technologists (India) 2021.