Yu Sun1,2, Xiao-Lin Qi2,3, Xiao Lin4, Xiao-Yu Zhang2, Li Gao2, Hua Gao2,3. 1. Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250117, Shandong Province, China. 2. Eye Hospital of Shandong First Medical University (Shandong Eye Hospital), Jinan 250021, Shandong Province, China. 3. State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong First Medical University & Shandong Academy of Medical Sciences, School of Ophthalmology, Shandong First Medical University, Jinan 250021, Shandong Province, China. 4. Eye Hospital Affiliated to Shandong University of Chinese Medicine, Jinan 250004, Shandong Province, China.
Abstract
AIM: To describe the design and clinical application of a corneal donor dehydrator which can quickly dehydrate corneas and keep its original shape. METHODS: The corneal donor material is placed on stainless steel beads with different diameters in the dehydrating box to make the cornea the same shape as the steel ball. Then, the cornea is placed inside the dehydrater for rapid dehydrating using the internal cleaning and ventilation system. Totally 83 eyes underwent deep anterior lamellar keratoplasty (DALK) using corneal donor tissue preserved with corneal dehydrater, and 60 patients (60 eyes) received DALK by the same surgeon using corneal donor tissue preserved with glycerol were included in the control group. The best corrected visual acuity (BCVA), the thickness and transparency of the corneal buttons were recorded. RESULTS: After the completion of dehydrating, all the donor corneas maintained a normal shape without any shrinkage or distortion, and the average intraoperative rehydration time was 43.3±12.1s during operation. The mean BCVA of the dehydrater group was 0.30±0.18 at 1wk and 0.32±0.16 at 1mo, which were statistically better than that of the control group (P<0.001). The score of corneal buttons transparency were lower than that of the control group with statistical difference (P<0.001). The thickness of corneal buttons at 1wk and at 1mo in the dehydrater group was significantly better than that of the control group respectively (P<0.001). One week after operation, no corneal button turbidity or edema was observed in both groups. CONCLUSION: The dehydrater can quickly dehydrate the corneal material in a clean and airtight environment and maintain the original shape of the corneal donor during the dehydrating process. This dehydrater is recommended for long-term high-quality preservation in areas where corneal materials cannot be used within a reasonable time period. International Journal of Ophthalmology Press.
AIM: To describe the design and clinical application of a corneal donor dehydrator which can quickly dehydrate corneas and keep its original shape. METHODS: The corneal donor material is placed on stainless steel beads with different diameters in the dehydrating box to make the cornea the same shape as the steel ball. Then, the cornea is placed inside the dehydrater for rapid dehydrating using the internal cleaning and ventilation system. Totally 83 eyes underwent deep anterior lamellar keratoplasty (DALK) using corneal donor tissue preserved with corneal dehydrater, and 60 patients (60 eyes) received DALK by the same surgeon using corneal donor tissue preserved with glycerol were included in the control group. The best corrected visual acuity (BCVA), the thickness and transparency of the corneal buttons were recorded. RESULTS: After the completion of dehydrating, all the donor corneas maintained a normal shape without any shrinkage or distortion, and the average intraoperative rehydration time was 43.3±12.1s during operation. The mean BCVA of the dehydrater group was 0.30±0.18 at 1wk and 0.32±0.16 at 1mo, which were statistically better than that of the control group (P<0.001). The score of corneal buttons transparency were lower than that of the control group with statistical difference (P<0.001). The thickness of corneal buttons at 1wk and at 1mo in the dehydrater group was significantly better than that of the control group respectively (P<0.001). One week after operation, no corneal button turbidity or edema was observed in both groups. CONCLUSION: The dehydrater can quickly dehydrate the corneal material in a clean and airtight environment and maintain the original shape of the corneal donor during the dehydrating process. This dehydrater is recommended for long-term high-quality preservation in areas where corneal materials cannot be used within a reasonable time period. International Journal of Ophthalmology Press.
Entities:
Keywords:
corneal donor; dehydration; eye bank; keratoplasty; organ culture storage