Literature DB >> 3558827

The use of 3,3',5,5'-tetramethylbenzidine as a peroxidase substrate in microplate enzyme-linked immunosorbent assay.

A K Goka, M J Farthing.   

Abstract

The assay conditions for the use of 3,3',5,5' tetramethylbenzidine (TMB) in microplate enzyme-linked immunosorbent assay are described. TMB is a safe (non-mutagenic) chromogen that is more sensitive than OPD as a substrate for horseradish peroxidase. We describe the optimum storage and assay conditions for this chromogen.

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Year:  1987        PMID： 3558827     DOI： 10.1080/01971528708063053

Source DB:  PubMed          Journal:  J Immunoassay        ISSN： 0197-1522


  9 in total

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2.  Non-specific reactions in enzyme linked immunosorbent assays for serum antibody to entamoeba histolytica and Giardia lamblia in non-endemic areas.

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3.  Low gluten diet in the treatment of adult coeliac disease: effect on jejunal morphology and serum anti-gluten antibodies.

Authors:  A M Montgomery; A K Goka; P J Kumar; M J Farthing; M L Clark
Journal:  Gut       Date:  1988-11       Impact factor: 23.059

4.  An Ultrasensitive and Selective Determination of Cadmium Ions at ppt Level Using an Enzymic Membrane with Colorimetric and Electrochemical Detection.

Authors:  Raouia Attaallah; Aziz Amine
Journal:  Biosensors (Basel)       Date:  2022-05-07

5.  Role of prostanoids in the inflammatory reaction and their therapeutic potential in the skin.

Authors:  R Ekerdt; B Müller
Journal:  Arch Dermatol Res       Date:  1992       Impact factor: 3.017

6.  Decorin potentiates interferon-γ activity in a model of allergic inflammation.

Authors:  Carla Bocian; Ann-Kathrin Urbanowitz; Rick T Owens; Renato V Iozzo; Martin Götte; Daniela G Seidler
Journal:  J Biol Chem       Date:  2013-03-04       Impact factor: 5.157

7.  Enhanced Colorimetric Signal for Accurate Signal Detection in Paper-Based Biosensors.

Authors:  Dorin Harpaz; Evgeni Eltzov; Timothy S E Ng; Robert S Marks; Alfred I Y Tok
Journal:  Diagnostics (Basel)       Date:  2020-01-07

8.  Development of an Inhibition Enzyme-Linked Immunosorbent Assay (ELISA) Prototype for Detecting Cytotoxic Three-Finger Toxins (3FTxs) in African Spitting Cobra Venoms.

Authors:  Ernest Z Manson; Kyama C Mutinda; Joseph K Gikunju; Aleksandra Bocian; Konrad K Hus; Vladimír Petrílla; Jaroslav Legáth; James H Kimotho
Journal:  Molecules       Date:  2022-01-28       Impact factor: 4.411

9.  Capacitive Saccharide Sensor Based on Immobilized Phenylboronic Acid with Diol Specificity.

Authors:  Gizem Ertürk Bergdahl; Martin Hedström; Bo Mattiasson
Journal:  Appl Biochem Biotechnol       Date:  2018-10-28       Impact factor: 2.926
  9 in total

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