Carlos Vinicius Barros Oliveira1, Daniel Honorato Neves2, Elayne Eally de Souza Morais2, Thalyta Julyanne Silva de Oliveira3, Mayara Maria da Silva4, Luiz Marivando Barros3, Antonia Eliene Duarte2. 1. Laboratory of Biology and Toxicology (BIOTOX), Department of Biology, Center for Biological and Health Sciences, Regional University of Cariri-URCA, CNPJ-06.740.864/0001-26, CEL. Antônio Luiz Street, 1161-Pimenta, Crato, CE, CEP: 63105-000, Brazil. viniciusbluesky@gmail.com. 2. Laboratory of Biology and Toxicology (BIOTOX), Department of Biology, Center for Biological and Health Sciences, Regional University of Cariri-URCA, CNPJ-06.740.864/0001-26, CEL. Antônio Luiz Street, 1161-Pimenta, Crato, CE, CEP: 63105-000, Brazil. 3. Laboratory of Plant Ecophysiology (LECOV), Department of Biology, Center for Biological and Health Sciences, Regional University of Cariri-URCA, CNPJ-06.740.864/0001-26, CEL. Antônio Luiz Street, 1161-Pimenta, Crato, CE, CEP: 63105-000, Brazil. 4. Semi-Arid Bioprospecting Laboratory and Alternative Methods (LABSEMA), Department of Biology, Center for Biological and Health Sciences, Regional University of Cariri-URCA, CNPJ-06.740.864/0001-26, CEL. Antônio Luiz Street, 1161-Pimenta, Crato, CE, CEP: 63105-000, Brazil.
Abstract
INTRODUCTION: The lobster cockroach Nauphoeta cinerea (N. cinerea) is indicated as a promising non-mammalian model, because it presents behavioral and biochemical alterations also observed in conventional models. In this research, we identified and characterized the distribution of protozoa that inhabit the digestive system (DS) of N. cinerea cockroaches. METHODS: The adult specimens of N. cinerea used in this study (n = 32) were obtained at the Federal University of Santa Maria, dissected and had their visceral contents observed in bright-field microscopy without staining and after application of lugol, Ziehl-Neelsen staining, EA36 trichrome and simulated dark-field microscopy with application of nankin ink. The presence of protozoa in different portions of the DS was semi-quantified by a system of crosses (+). RESULTS: The main taxa observed were: amoebas (Archaemebae:Entamoebida), gregarins (Apicomplexa:Eugregarinide), coccidia (Apicomplexa:Eucoccidiorida), kinetoplastids (Kinetoplastea:Kinetoplastida) and oxymonads (Preaxostyla:Oxymonadida). The highest prevalence of amoebas and gregarines was observed in the medial portion of the DS, while for the other groups, this was seen in the final portion, and in the case of coccidia, such prevalence was specially evidenced by the alcohol-acid coloration. In the present work, the great biological diversity that exists in the microbiota of the digestive system of Nauphoeta cinerea was demonstrated, being possible to find several pathogenic species for humans such as Entamoeba histolytica/dispar/moshkovskii, Cryptosporidium sp. and Cyclospora cayetanensis. There is still a lot to know about the interactions between endocommensal protozoa and their respective invertebrate hosts, so the best way to clarify such relationships is through molecular and genetic test.
INTRODUCTION: The lobster cockroach Nauphoeta cinerea (N. cinerea) is indicated as a promising non-mammalian model, because it presents behavioral and biochemical alterations also observed in conventional models. In this research, we identified and characterized the distribution of protozoa that inhabit the digestive system (DS) of N. cinerea cockroaches. METHODS: The adult specimens of N. cinerea used in this study (n = 32) were obtained at the Federal University of Santa Maria, dissected and had their visceral contents observed in bright-field microscopy without staining and after application of lugol, Ziehl-Neelsen staining, EA36 trichrome and simulated dark-field microscopy with application of nankin ink. The presence of protozoa in different portions of the DS was semi-quantified by a system of crosses (+). RESULTS: The main taxa observed were: amoebas (Archaemebae:Entamoebida), gregarins (Apicomplexa:Eugregarinide), coccidia (Apicomplexa:Eucoccidiorida), kinetoplastids (Kinetoplastea:Kinetoplastida) and oxymonads (Preaxostyla:Oxymonadida). The highest prevalence of amoebas and gregarines was observed in the medial portion of the DS, while for the other groups, this was seen in the final portion, and in the case of coccidia, such prevalence was specially evidenced by the alcohol-acid coloration. In the present work, the great biological diversity that exists in the microbiota of the digestive system of Nauphoeta cinerea was demonstrated, being possible to find several pathogenic species for humans such as Entamoeba histolytica/dispar/moshkovskii, Cryptosporidium sp. and Cyclospora cayetanensis. There is still a lot to know about the interactions between endocommensal protozoa and their respective invertebrate hosts, so the best way to clarify such relationships is through molecular and genetic test.
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Authors: Jessica R Deere; Michele B Parsons; Elizabeth V Lonsdorf; Iddi Lipende; Shadrack Kamenya; D Anthony Collins; Dominic A Travis; Thomas R Gillespie Journal: Parasitology Date: 2018-08-30 Impact factor: 3.234
Authors: Z N Tan; W K Wong; Z Nik Zairi; B Abdullah; N Rahmah; M Zeehaida; S Rumaizi; P Lalitha; G C Tan; A Olivos-Garcia; B H Lim Journal: Trop Biomed Date: 2010-04 Impact factor: 0.623
Authors: N R Rodrigues; M E M Nunes; D G C Silva; A P P Zemolin; D F Meinerz; L C Cruz; A B Pereira; J B T Rocha; T Posser; J L Franco Journal: Chemosphere Date: 2013-03-07 Impact factor: 7.086