Preparative low-pressure chromatography of antibiotics on a column of diol-bonded silica gel.

Several schemes are presented which illustrate the general utility of column chromatography on diol-bonded silica gel for the purification of various antibiotics. The antibiotics include rosarimicin, rosarimicin dimethylacetal, everninomicin D, Juvenimicin A4, coloradocin and the benzanthrins. A set of partition coefficients, determined in different two-phase solvent systems, for a given antibiotic or antibiotic complex, can be used in selecting appropriate solvent systems for this chromatography as well as for semi-preparative counter-current chromatography on the Ito coil planet centrifuge.