Simultaneous quantification of myocardial adenine nucleotides and creatine phosphate by ion-pair reversed-phase high-performance liquid chromatography.

A new ion-pair reversed-phase high-performance liquid chromatography method was applied to simultaneous measurements of porcine myocardial adenine nucleotides and creatine phosphate. The homogeneity of each desired peak was tested by the retention times of standards, chromatography of spiked samples, the absorbance ratios at various wavelengths, and the differing retention times for a number of other compounds found in porcine myocardial extracts.