Literature DB >> 35585317

Purification and Analysis of Nucleotides and Nucleosides from Plants.

Henryk Straube1, Marco Herde2.   

Abstract

This protocol describes necessary steps to isolate and quantify nucleotides and nucleosides from plant samples. Proper sample preparation in combination with liquid chromatography coupled to mass spectrometry enables the sensitive detection and quantification of metabolites of low abundance. Utilizing a liquid-liquid extraction in combination with a weak anion-exchange solid phase extraction enables the separation of negatively charged molecules from uncharged metabolites or cations.
© 2022. Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Liquid chromatography; Mass spectrometry; Metabolomics; Plant nucleotide metabolism; Polar metabolomics; Weak anion-exchange solid phase extraction

Mesh：

Substances：

Year:  2022        PMID： 35585317     DOI： 10.1007/978-1-0716-2176-9_11

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  8 in total

1.  THE PROBLEM OF HALTING ENZYME ACTION WHEN EXTRACTING PLANT TISSUES.

Authors:  R L BIELESKI
Journal:  Anal Biochem       Date:  1964-12       Impact factor: 3.365

2.  Arabidopsis ribonucleotide reductases are critical for cell cycle progression, DNA damage repair, and plant development.

Authors:  Chunxin Wang; Zhongchi Liu
Journal:  Plant Cell       Date:  2006-01-06       Impact factor: 11.277

3.  Structure and function of nucleoside hydrolases from Physcomitrella patens and maize catalyzing the hydrolysis of purine, pyrimidine, and cytokinin ribosides.

Authors:  Martina Kopecná; Hanna Blaschke; David Kopecny; Armelle Vigouroux; Radka Koncitíková; Ondrej Novák; Ondrej Kotland; Miroslav Strnad; Solange Moréra; Klaus von Schwartzenberg
Journal:  Plant Physiol       Date:  2013-10-29       Impact factor: 8.340

4.  Rice virescent3 and stripe1 encoding the large and small subunits of ribonucleotide reductase are required for chloroplast biogenesis during early leaf development.

Authors:  Soo-Cheul Yoo; Sung-Hwan Cho; Hiroki Sugimoto; Jinjie Li; Kensuke Kusumi; Hee-Jong Koh; Koh Iba; Nam-Chon Paek
Journal:  Plant Physiol       Date:  2009-03-18       Impact factor: 8.340

5.  Of the Nine Cytidine Deaminase-Like Genes in Arabidopsis, Eight Are Pseudogenes and Only One Is Required to Maintain Pyrimidine Homeostasis in Vivo.

Authors:  Mingjia Chen; Marco Herde; Claus-Peter Witte
Journal:  Plant Physiol       Date:  2016-03-31       Impact factor: 8.340

6.  Plant purine nucleoside catabolism employs a guanosine deaminase required for the generation of xanthosine in Arabidopsis.

Authors:  Kathleen Dahncke; Claus-Peter Witte
Journal:  Plant Cell       Date:  2013-10-15       Impact factor: 11.277

7.  crinkled leaves 8--a mutation in the large subunit of ribonucleotide reductase--leads to defects in leaf development and chloroplast division in Arabidopsis thaliana.

Authors:  Sarah Garton; Heather Knight; Gareth J Warren; Marc R Knight; Glenn J Thorlby
Journal:  Plant J       Date:  2007-03-05       Impact factor: 6.417

8.  Enhanced nucleotide analysis enables the quantification of deoxynucleotides in plants and algae revealing connections between nucleoside and deoxynucleoside metabolism.

Authors:  Henryk Straube; Markus Niehaus; Sarah Zwittian; Claus-Peter Witte; Marco Herde
Journal:  Plant Cell       Date:  2021-04-17       Impact factor: 11.277
  8 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.