Literature DB >> 35582063

SIBLING PAIR ANALYSIS IN THE IDENTIFICATION PROCESS OF THE MADURESE POPULATION USING STR CODIS LOCI.

Ahmad Yudianto1,2,3, Arofi Kurniawan4,2, Fery Setiawan2,5, Egita Windrianatama Puspa2, Racy Youngest2.   

Abstract

Background: The most common problem in forensic personal identification, particularly in paternity tests, is the availability of information originating from a mother or a father that can be used as a comparison in the forensic DNA examination process. The use of sibling analysis in paternity tests is still not widely known. Materials and methods: The respondents of this study were 25 families of the Madurese population with 6, 9, and 10 sibling pairs categorized as female-female, male-male, and male-female siblings. The kinship relationship was analyzed by using 13 STR CODIS loci technique (TPOX, D3S1358, FGA, D5S818, CSFIPO, D7S820, D8S1179, THOI, vWA, D13S317, D16S539, D18S51, D21S11), and Amelogenin (Amel) x: 106bp,y: 112bp).
Results: The results of DNA contents and purity examination of the extracted DNA sample showed that the average value of DNA contents was 675±5.35 ng/μL with a purity range of 1.05-1.86. The findings proved that the male-male siblings of the Madurese population had the highest allele sharing percentage at the loci of D13S317, D16S539, and D21S11. The highest allele sharing percentage for female-female siblings was at the loci of TPOX and D21S11. Meanwhile, the male-female siblings had the highest allele sharing percentage at the loci of TPOX, D5S818, vWA, D7S820, THO1, vWA, and D13S317.
Conclusion: The main STR loci recommended in the male-female siblings of Madurese population identification are TPOX, D13S317, and D21S11. Copyright:
© 2022 Afr. J. Infect. Diseases.

Entities:  

Keywords:  Human &; Identification; Madurese Population; Mortality; STR CODIS; Siblings

Year:  2022        PMID： 35582063      PMCID： PMC9097306          DOI： 10.21010/Ajid.v16i2.5

Source DB:  PubMed          Journal:  Afr J Infect Dis        ISSN： 2006-0165


Introduction

A paternity test is a means to determine whether a man is a biological father. It is a valid legal procedure for determining paternity. Paternity determination is very complicated because many aspects need to be examined. Until now, the solution for paternity problems is assessed by examining the similar or dissimilar features of a child and the alleged father. The similar features here refer to characteristics of irises’ color of the eyes, hair, unique manners or way of speaking, and stature (Yudianto, 2015: Yudianto et al., 2021). The most common challenge faced during the paternity test process is the unavailability of genetic information from the parents. The unavailability of genetic information from both parents as a comparison in an identification process is one of the problems in forensic DNA analysis, particularly in paternity tests (Sykuriani, 2012: Yudianto, 2013). This condition requires a comparison that has a close family line as a solution in the process of forensic DNA analysis such as biological siblings (Gaytmenn et al., 2002). The forensic identification process of siblings is also conducted through the examination of alleles at the STR loci of nuclear DNA. Based on this reason, the use of siblings as the comparison through STR remains unknown in Indonesia as well as other countries regardless of the demand for ethnic and population diversity, natural disasters, and other events that require forensic readiness. The application usually targets the use of human profiling and typing, forensic application, sibship relationship, craniology tracing or individual identification, and incestuous identification (Consetino et al., 2015: Karbeyas K et al., 2016; Yudianto et al., 2021). The Madurese are one of the ethnic groups with the highest population in Indonesia. Based on National Census 2010, the Madurese population was 7,179,356. They are originating from the island of Madura and its nearby islets (Puteran, Gili Iyang, Sapudi, Gili Raja, Gili Genting, Raas, etc). Additionaly, they are a wandering tribe that can be found in many places across Indonesia. The population can also be found in neighboring countries, such as Malaysia and Singapore. In general, the Madurese population was chosen in this study because of their unique characteristics in terms of their temperament and unique accents. Most of the Madurese population have strong work ethics and are adventurous. These characteristics make Madurese people tend to migrate from their homeland (Prastowo W et al, 2018; Sosiawan A et al., 2019).

Materials and methods

Population and Research Sample

The population of this research was all subjects undergoing paternity examination at Human Genetic Study Group, The Institute of Tropical Disease, Universitas Airlangga. The research sample was originating from the peripheral blood of paternity examination respondents, consisting of a father, a mother, and two children of the Madurese population. The father and the mother serve as the control/reference for allele sharing between the siblings. The inclusion criterion for this research was a family consisting of at least three generations of pure Madurese population with two biological children. This research has obtained ethical clearance from the Faculty of Dentistry Universitas Airlangga Number 275/HRECC.FODM/VI/2020. The number of respondents in this study was 25 with 100 samples. This research was conducted at the Human Genetic Study Group, the Institute of Tropical Disease Universitas Airlangga.

Sample Preparation

One hundred peripheral blood samples of the respondents were stored inside tubes and labeled as A [father], B [mother], and C [children]. The labels were used to indicate that the samples were collected from fathers, mothers, and biological children.

DNA Extraction

The DNA extraction process of 100 peripheral blood samples was carried out using the DNAzol method (McClintock 2014). 50 μL of distilled water was added to the isolated DNA pellet.

DNA Amplification

The DNA amplification process was conducted through Polymerase Chain Reaction (PCR) process (PowerPlex® 21Systems, Promega, USA) targeting specific DNA sequences to replicate the isolated DNA. The amplification of 80 samples was conducted using 13 Short Tandem Repeats [STR]-Combined DNA Index System [CODIS] (TPOX, D3S1358, FGA, D5S818, CSFIPO, D7S820, D8S1179, THOI, vWA, D13S317, D16S539, D18S51, D21S11) and Amelogenin (Amel) x:106bp,y:112bp. The amplification adjustment for D3S1358, FGA, D8S1179, D18S51, and D21S11 was: 96 °C- 2 minutes, followed by [ 94 °C-1 minute, 60 °C-1 minute, 70 °C-1.5 minutes for 10 cycles], [90 °C-1 minute, 64 °C- 1 minute, 70 °C-1.5 minutes for 25 cycles]. The adjustment for CSF1PO was 96 °C-2 minutes, followed by [94 °C-1 minute, 64 °C-1 minute, 70 °C-1.5 minutes for 10 cycles], [90 °C-1 minute, 64 °C- 1 minute, 70 °C-1.5 minutes for 30 cycles]. The amplification adjustment for D5S818, D7S820, and D13S317 was 96 °C- 1 minute, followed by [94 °C-30 seconds, 60 °C-30 seconds, 70 °C-45 seconds for 10 cycles], [90 °C-30 seconds, 64 °C- 30 seconds, 70 °C-45 seconds for 30 cycles]. The amplification adjustment for D16S539 was 96 °C-1 minute, followed by [94 °C-1 minute, 59 °C-1 minutes, 72 °C-1.5 minutes for 25 cycles], and 72 °C-1 minutes. All DNA templates were stored at 4 °C temperature (Promega corp, 2001).

Electrophorese Gel

The visualization of PCR results was conducted through vertical electrophoresis using 6% polyacrylamide agarose gel [PAGE] [Bio-Rad Mini-PROTEAN®] with silver nitrate staining (Figure 1).
Figure 1

PCR visualization of CSF1PO locus [321bp-357bp]. M (Marker), A (Father), B (Mother), Ca (Child 1), Cb (Child 2)

PCR visualization of CSF1PO locus [321bp-357bp]. M (Marker), A (Father), B (Mother), Ca (Child 1), Cb (Child 2)

Sample Alleles

The reading of PCR visualization on the electrophoresis gel resulted in alleles at each locus with K562 as the control (Table I). The alleles were matched with family members (the father, the mother, and the children) and the value of allele frequency (Table II). The analysis was based on the frequency of allele sharing of kinship analysis between biological siblings at every STR CODIS locus by examining the allele sharing (Figure 2, 3, 4, 5).
Table 1

STR Allele Profile of the 25 Families

STR CODIS
FamilyCodeTPOXD3 S1358FGAD5 S818CSF1P0D7 S820D8 S1179THO1vWAD13 S317D16 S539D18 S51D21S11Amel
1A18,916,1721,2411,129,109,1112,138,915,188,811,1115,1629,31106,112
B18,1016,1621,2411,128,109,1112,139,1016,178,811,1115,1630,31106
C1a8,816,1621,2112,128,109,1112,129,1015,178,811,1115,1630,31106,112
C1b8,916,1621,2411,129,109,1112,139,1015,178,811,1115,1531.31106,112
2A29,1015,1721,2210,129,108,811,1211,1319,208,910,1114,1529,31106,112
B28,916,1721,249,108,99,1010,119,1018,198,1011,1315,1530,31106
C2a8,917,1721,2110,109,98,911,119,1119,198,811,1115,1531,31106
C2b8,915,1721,2210,128,98,911,119,1119,198,911,1115,1531,31106
3A38,915,1720,249,118,88,99,108,915,178,119,1114,1629,32106,112
B39,1016,1722,2411,119,109,1011,128,1018,199,109,1016,1630,32106
C3a9,1016,1724,2411,118,99,910,118,817,189,119,916,1632.32106
C3b9,1016,1724,2411,118,98,910,128,917,188,99,916,1629,30106
4A49,916,1822,248,99,108,119,1211,1320,219,1110,1115,1631,32106,112
B49,1017,1821,2210,129,119,1012,1311,1216,189,1110,1016,1729,31106
C4a9,918,1822,229,109,99,1112,1211,1116,209,910,1016,1631,31106,112
C4b9,918,1821,249,109,118,912,1211,1116,209,910,1016,1631,31106
5A58,1016,1720,2111,138,129,1110,128,1216,179,119,1114,1729,31106,112
B58,917,1722,249,119,109,119,129,1118,2011,118,1016,1730,31106
C5a8,817,1721,2211,119,129,912,128,916,1811,118,914,1631,31106
C5b8,817,1721,2211,119,129,912,128,916,1811,118,917,1731,31106
6A67,1015,1821,219,118,98,1011,128,919,208,911,1314,1529,31106,112
B610,1116,1722,2311,1110,119,1112,1310,1318,1910,1210,1215,1530,31106
C6a7,1116,1821,2211,118,118,912,128,1019,199,1011,1215,1530,31106,112
C6b10,1116,1821,2311,118,119,1012,128,1019,199,1011,1215,1531.31106,112
7A79,1016,1720,2110,1210,118,1111,1311,1217,198,811,1114,1629,31106,112
B78,915,1722,2411,139,109,1010,129,1017,208,910,1016,1630,31106
C7a8,917,1721,2211,1210,109,1111,129,1117,178,810,1116,1631,31106
C7b8,915,1721,2211,129,118,911,129,1117,198,810,1116,1631,31106,112
8A89,1017,1922,2410,129,119,1112,1311,1215,178,99,1114,1731,32106,112
B88,916,1821,239,1110,119,1112,1310,1120,218,108,1016,1729,31106
C8a8,917,1821,249,109,119,912,1211,1115,218,88,914,1631,31106,112
C8b8,917,1821,249,109,119,912,1311,1115,218,98,917,1731,31106,112
9A911,1316,1822,2311,128,109,1111,128,1316,179,1111,1314,1529,31106,112
B911,1217,1721,2410,119,109,1112,139,1018,209,1110,1215,1530,31106
C9a11,1116,1721,2411,118,99,912,128,916,189,911,1215,1531,31106
C9b11,1116,1721,2411,118,99,912,128,916,189,911,1215,1531,31106,112
10A1010,1115,1720,249,118,129,1111,138,1115,189,1110,1115,1631,32106,112
B109,1016,1819,2211,138,1011,1110,128,916,1711,1110,1016,1729,31106
C10a10,1015,1820,2211,118,811,1111,128,815,1711,1110,1016,1631,31106,112
C10b9,1116,1720,229,138,811,1111,128,815,1711,1110,1016,1631,31106,112
11A1111,1315,1719,2110,129,108,812,138,915,179,119,1114,1729,31106,112
B1111,1216,1821,239,119,118,812,1310,1318,1911,118,1016,1730,31106
C11a11,1115,1821,219,109,98,812,128,1017,1811,118,914,1631,31106,112
C11b11,1116,1721,219,109,118,812,138,1017,1811,118,917,1731,31106
12A1211,1316,1720,2410,129,118,912,1311,1220,218,811,1314,1529,31106,112
B1211,1216,1619,229,1110,118,1012,139,1016,188,810,1215,1530,31106
C12a11,1116,1620,229,109,118,812,129,1116,208,811,1215,1530,31106
C12b11,1116,1620,229,109,118,912,139,1116,208,811,1215,1531.31106
13A139,1016,1722,2410,128,108,1111,128,1315,188,910,1115,1629,31106,112
B138,917,1721,239,109,109,1012,139,1016,178,1010,1016,1730,31106
C13a8,917,1721,2410,108,99,1112,128,915,178,810,1016,1631,31106,112
C13b8,917,1721,2410,128,98,912,128,915,178,910,1016,1631,31106,112
14A148,915,1822,239,118,129,1111,138,1116,178,89,1114,1729,31106,112
B149,1016,1721,2411,118,109,1110,128,918,208,88,1016,1730,31106
C14a9,1016,1821,2411,118,89,911,128,816,188,88,914,1630,31106,112
C14b9,1016,1821,2411,118,89,911,128,816,188,88,917,1731.31106
15A159,917,1921,248,99,109,1112,1311,1315,189,119,1114,1728,30106,112
B159,1016,1821,2410,129,1111,1112,1311,1216,179,118,1016,1730,31106
C15a9,917,1821,219,109,911,1112,1211,1115,179,98,914,1630,30106,112
C15b9,917,1821,249,109,1111,1112,1311,1115,179,98,917,1730,31106
16A169,1016,1821,228,98,128,911,128,1219,209,1110,1115,1631,32106,112
B168,917,1721,2410,128,108,1010,119,1118,1911,1110,1016,1729,31106
C16a8,916,1721,219,108,88,811,118,919,1911,1110,1016,1631,31106,112
C16b8,916,1721,229,108,88,911,118,919,1911,1110,1016,1631,31106,112
17A1711,1315,1720,2410,129,109,119,1011,1317,198,89,1114,1729,31106,112
B1711,1216,1819,229,109,119,1111,129,1017,208,88,1016,1730,31106,
C17a11,1115,1820,2210,109,99,910,119,1117,178,88,914,1631,31106
C17b11,1116,1720,2210,129,119,910,129,1117,198,88,917,1731,31106,112
18A1810,1116,1722,248,98,129,1111,138,916,178,911,1314,1529,31106,112
B189,1016,1621,2310,129,1011,1110,129,1018,208,1010,1215,1530,31106
C18a10,1016,1621,249,109,1211,1111,129,1016,188,811,1215,1530,31106,112
C18b9,1116,1621,249,109,1211,1111,129,1016,188,911,1215,1531.31106,112
19A1911,1315,1821,249,118,129,1112,1311,1319,208,119,1114,1731,32106,112
B1911,1216,1721,2411,138,1011,1112,139,1018,199,108,1016,1729,31106
C19a11,1116,1821,2111,118,811,1112,129,1119,199,118,914,1631,31106
C19b11,1116,1821,249,138,811,1112,139,1119,198,98,917,1731,31106,112
20A209,917,1921,2210,129,108,811,128,1317,198,911,1314,1529,31106,112
B209,1016,1821,249,119,118,810,119,1017,208,1010,1215,1530,31106
C20a9,917,1821,219,109,98,811,118,917,178,811,1215,1531,31106,112
C20b9,917,1821,229,109,118,811,118,917,198,811,1215,1531,31106,112
21A208,917,1821,2210,119,108,811,118,1117,198,911,1114,1529,30106,112
B209,1016,1821,249,119,117,810,119,1017,208,1010,1215,1530,31106
C20a9,917,1821,219,109,98,811,118,1117,178,811,1215,1530,31106
C20b9,917,1821,229,119,117,811,118,817,198,1011,1215,1531,31106
22A2011,1317,1721,2410,129,107,811,128,1317,188,911,1314,1529,31106,112
B2011,1216,1821,249,119,118,810,119,1117,198,1010,1215,1530,31106
C20a11,1117,1821,219,109,97,811,118,917,178,911,1215,1530,31106,112
C20b11,1117,1821,249,109,118,811,118,917,198,811,1215,1431,31106
23A2010,1117,1920,2210,129,108,811,128,917,208,1111,1214,1529,31106,112
B209,1016,1820,239,119,118,810,119,1017,208,1010,1215,1530,31106
C20a10,1017,1820,209,109,98,811,118,917,178,811,1215,1531,31106,112
C20b9,1117,1820,229,109,118,811,118,917,208,1111,1215,1531,31106,112
24A209,1116,1921,2310,1210,108,811,118,1317,198,911,1214,1529,30106,112
B209,1016,1821,249,119,118,810,109,1018,208,1010,1215,1630,31106
C20a9,916,1821,239,109,108,811,108,917,188,811,1215,1630,31106
C20b9,916,1821,219,109,108,811,108,917,188,811,1214,1530,30106,112
25A209,1018,1921,2210,119,108,811,128,1117,198,1111,1315,1529,30106,112
B209,1018,1821,239,109,118,810,129,1017,208,1010,1115,1530,31106
C20a9,1018,1821,219,109,98,811,128,817,178,811,1115,1529,30106
C20b10,1018,1821,229,109,108,812,128,1017,198,1111,1115,1530,31106
Table 2

Frequencies of STR Alleles of the Sample (N=200)

AlleleFrequenciesAlleleFrequencies
TPOX THOI
70,0125080,25250
80,1750090,27000
90,34375100,10750
100,18125110,27000
110,22500120,05000
120,03125130,05000
130,03125
D3S1358 vWA
150,09375150,12750
160,33125160,10750
170,35000170,19500
180,20625180,22250
190,01875190,19500
200,10250
210,05000
FGA D13S317
190,0375080,43750
200,0875090,25625
210,35625100,06250
220,22500110,23750
230,05000120,00625
240,24375
D5S818 D16S539
80,0250080,13125
90,2125090,16875
100,25625100,29375
110,32500110,28125
120,14375120,08750
130,03750130,03750
CSF1PO D18S51
80,23250140,13750
90,35750150,31875
100,20250160,34375
110,15750170,20000
120,05000
D8S1179 D21S11
90,02500280,00625
100,08750290,12500
110,23125300,16875
120,50000310,64375
130,15625320,05625
D7S820
80,25000
90,38125
100,05625
110,3125
Figure 2

Allele Sharing Percentage

Figure 3

Sibling Percentage Based on Sex

Figure 4

Percentage of Allele Sharing on Siblings

Figure 5

Percentage of STR loci allele sharing on female-female siblings

STR Allele Profile of the 25 Families Frequencies of STR Alleles of the Sample (N=200) Allele Sharing Percentage Sibling Percentage Based on Sex Percentage of Allele Sharing on Siblings Percentage of STR loci allele sharing on female-female siblings

Results

The measurement of DNA contents and DNA purity examination of the extracted DNA samples results in the average value of DNA contents of 675 ± 5.35 ng/μl with a purity range of 1.05-1.86. The PCR amplification was carried out through 13 STR CODIS loci. The visualization of PCR results was carried out through Polyacrylamide Agarose Gel Electrophorese (PAGE) with silver nitrate staining. The image of PCR results visualization is presented in Figure 1 below: Table 1 above shows that 50 % of the genetic materials of an individual are inherited from each side of the parents. Since the nuclear DNA (nDNA) of an individual is inherited from both parents, it can be said that the inheritance of nuclear DNA follows the Mendelian way. The First Mendelian Law (Segregation of allelic genes) states the principles of allelic segregation during gamete formation. Gamete formation occurs through meiosis where the homologous pairs are decoupling and segregated. The alleles of a gene freely segregate from diploid to haploid (Elvita A et al., 2008: Mangoendidjojo W, 2014: Artadana IBM et al., 2018). This finding indicates that the percentage of male-male siblings is 24%; female-female siblings is 36%, and male-female siblings is 40% (Figure 2 and Figure 3). Meanwhile the alleles of STR CODIS loci with the highest percentage are: TPOX allele 9 [34.375%], D3S1358 allele 17 [35%], FGA allele 21 [35.625%], D5S818 allele 11 [32.5%], CSF1PO allele 9 [35.75%], DS820 allele 9 [38.12%], D8S1179 allele 12 [50%], THOI allele 9 [27%], vWA allele 18 [22.25%], D13S317 allele 8 [43.75%], D16S539 allele 10 [29.375%], D18S51 allele 16 [34.375%], and D21S11 allele 31 [64.375%] (Table 2). Figure 4 shows that D7S820, D8S1179, THO1, vWA, D13S317, and D16S539 with zero allele sharing are found in the samples while the highest percentage is found on two alleles sharing of TPOX and vWA loci (80%). In Figure 5 describing female-female siblings, the percentage of two alleles sharing at D21S11 locus is 85 %, and there is 0 (zero) allele sharing on all the examined loci. The male-male siblings’ group shows that D13S317 locus has two alleles sharing (100%) while CSF1PO, D7S820, D8S1179, THO1, vWA, D16S539, and D21S11 loci have 0 (zero) allele sharing (0%) (Figure 6). In male-female siblings, the loci D7S820, D8S1179, THO1, vWA, D13S317, and D16S539 with zero allele sharing is 0% (Figure 7).
Figure 6

Percentage of STR loci allele sharing on male-male siblings

Figure 7

Percentage of STR loci allele sharing on male-female siblings

Percentage of STR loci allele sharing on male-male siblings Percentage of STR loci allele sharing on male-female siblings The calculation of the Sibship Index (SI) of this study is presented in Table III below:
Table 3

Sibship Index Calculation

Sibship indices (SI) Probability RatioStrengthPercentage
< 1Weak10%
1 - 10Moderate10%
10 -100Strong25%
>100Very Strong55%
Sibship Index Calculation Table III displaying the result of sibling indices (SI) calculation shows that 55% of sibling pairs have SI higher than 100 (very strong) while 25% of sibling pairs have SI 10-100 (strong).

Discussion

This study indicates the basic principles applied in assessing the probability of general alleles in the examined sample and inherited reference of Identical by Descent (IBD) alleles. There is a 25% probability that two siblings inherit two IBD alleles from their parents, a 50% probability that the siblings inherit one IBD allele and a 25% probability that the siblings do not inherit any IBD allele from the parents. There is no shared allele on the diploid loci. The probability that the two siblings do not share any alleles (25%) at one of the two loci might cause significant issues in identifying individuals who die in mass disasters if there is only one biological sibling alive. The probability that one sibling inherits zero IBD allele from their parents is constant at 25% for every locus. The probability that two biological siblings do not share alleles/partial loci depends on polymorphism information contents (PIC) and heterozygosity (Lee et al., 2012). In genetics, alleles are the alternative forms of genes on specific loci that are related to the expression of certain characteristics (phenotype). Alleles are formed as the result of variations in nitrogen base sequences caused by mutation. In an individual, allele pairs determine the genotype of the individual. The term ‘allele’ exists as the result of the use of the term allelomorph in Mendel’s Principles of Heredity (Butler 2006: Elvita A et al., 2008: Butler et al., 2012). Individuals with the same alleles at a locus are called to have a homozygote genotype while individuals with different alleles are heterozygote genotypes. Since genotypes are expressed into certain phenotypes, alleles may cause different appearances among individuals within a population (Butler 2006: Butler 2015). In kinship examination using biological siblings, the sensitivity of variations depends on certainty thresholds. The higher the certainty threshold, the lower the sensitivity. High sensitivity is when the number of false-negative is low while high specificity is when the number of false-positive is low. Although biological siblings do not necessarily have the same alleles, allele sharing in biological siblings can be used to examine whether the siblings have kinship relationships as indicated in the study (Tzeng et al., 2000). Small variations in allelic distribution between siblings are also affected by endogamous marriage where the authors recommend the use of 25 loci for the Lebanese population (Setyowati et al., 2020). In forensic cases such as the identification of fire disaster victims, siblings are compared with the victims by utilizing the Y-chromosome to identify whether the siblings and the victims come from the same father (Maeda et al., 2015). Many previous studies have been conducted to assess the relationship between biological siblings mainly based on heterozygosity and combined sibship indices (CSIs) (Reid et al., 2004). A study utilizing STR to analyze genetic diversity in Indonesia has been conducted to examine the extent of genetic mixing between Javanese and Arab ethnic groups (Sari, 2017). A paternity test is a DNA test aimed to determine whether a man is the biological father of a child. Family dispute cases, in the form of doubting parents, are increasingly popular in Indonesian society (Syukriani Y, 2012). Therefore, a paternity test uses parents as the comparison where the results are statistically close to 100% or about 99.99% (Untoro et al., 2009). The unavailability of information originating from a father, a mother, or a child that can be used as a comparison in forensic DNA examination becomes a problem in forensic DNA examination (Jacewicz et al, 2003). Unlike DNA testing with parental DNA as the comparison, the accuracy rate of personal identifications using siblings’ information is not close to 100% (O Connor, 2011). The principle of identification examination through DNA is based on the process of allelic comparison between the victim or the perpetrator and the alleles from the family line, especially parents, following the Mendelian laws (Leclair B et al., 2004: Reid TM et al 2008: Hares DR, 2015). In case the parental or descendant line is unavailable, a comparison with a close family line is needed as a method taken in an identification examination through DNA, namely biological siblings. The use of siblings as the comparison is one of the identification methods known as kinship analysis. Similar to a paternity test, the kinship analysis in an identification process also has a possibility of mismatched profiles in the examined DNA loci (O’Connor, 2011; Mangoendidjojo W, 2014; Maeda K et al., 2015; Marano LA et al., 2019; Sosiawan et al., 2019). One of the aspects that need to be considered in assessing sibling relationships is minimizing the number of false-positive and increasing the number of the examined loci. The findings of this study show that male-male siblings of the Madurese population have a high percentage of allele sharing at D13S317, D16S539, and D21S11 loci while the female-female siblings at TPOX and D21S11 loci, and male-female siblings at TPOX, D5S818, vWA, D7S820, THO1, vWA, and D13S317 loci. These findings are beneficial in enriching the literature and the analysis of sibling relationships in Indonesian ethnic groups related to the paternity examination process or the identification of mass disaster victims.

Conclusion

This study signifies that two alleles sharing is stronger compared to one-allele sharing and zero-allele sharing. The 50% of two alleles sharing indicates that the interpretation of full siblings is accepted so that the relationship claim is true. When assessing full sibling relationships, careful consideration should be given to STR loci with high frequencies of two alleles sharing. The findings of this study show that male-male siblings of the Madurese population have a high percentage of allele sharing at D13S317, D16S539, and D21S11 loci while the female-female siblings at TPOX and D21S11 loci, and male-female siblings at TPOX, D5S818, vWA, D7S820, THO1, vWA, and D13S317 loci. The main STR loci recommended for male-female siblings of the Madurese population are TPOX, D13S317, and D21S11.

Conflict of Interest:

The authors declare that there is no conflict of interest in this study. Short Tandem Repeat Deoxyribonucleic Acid Combined DNA Index System Polymerase Chain Reaction Amelogenin polyacrylamide agarose gel electrophoreses Sibship Indices
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5.  Selection and implementation of expanded CODIS core loci in the United States.

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Journal:  Forensic Sci Int Genet       Date:  2015-03-12       Impact factor: 4.882

6.  DNAzol: a reagent for the rapid isolation of genomic DNA.

Authors:  P Chomczynski; K Mackey; R Drews; W Wilfinger
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7.  Validation of a male-specific, 12-locus fluorescent short tandem repeat (STR) multiplex.

Authors:  Benjamin E Krenke; Lori Viculis; Melanie L Richard; Mechthild Prinz; Scott C Milne; Carll Ladd; Ann Marie Gross; Tanis Gornall; J Roger H Frappier; Arthur J Eisenberg; Charles Barna; Xavier G Aranda; Michael S Adamowicz; Bruce Budowle
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