Modelling of urea hydrolysis kinetics using genetic algorithm coupled artificial neural networks in urease immobilized magnetite nanoparticles.

The presence of urea in runoff from fertilized soil could be contributing to the growth of dangerous blooms. Enzymatic urea hydrolysis is a well-known outstanding process that, when integrated with nanotechnology, would be much more efficient. This research provides a novel perspective on magnetic nanobiocatalysts that reduce diffusion barriers in effective urea hydrolysis. Surprisingly, the model developed with the use of a Genetic Algorithm (GA) and an Artificial Neural Network (ANN) demonstrated that the system's diffusion restrictions were reduced. In order to forecast accurate outputs using artificial intelligence (AI), a neural network with one hidden layer and 20 neurons was built utilizing multilayer feed-forward network and showed highest output (diffusion co-efficient) with least mean square error (MSE). The diffusion coefficients of free urease, urease immobilized onto porous MNs (U-aMNs), and nanobiocatalyst, i.e. urease immobilized onto surface modified MNs (U-MNβ), were 1.9 × 10-17, 12.62 × 10-16, and 15.48 × 10-16 cm2/min, respectively. These results revealed that the addition of Chitosan to the surface of MNs had a considerable impact on enzyme dispersion. The decrease in Damkohler number (Da) from 2.37 ± 0.26 for U-aMNs to 2.19 ± 0.11 for U-MNβ suggested a beneficial effect in overcoming diffusion constraints. Pseudo-first order and pseudo-second order models were used to analyze urea uptake kinetics, with the former model offering the best fit to the system, with R2 values that were much closer to unity.